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Otx2 is expressed in the mesencephalon and prosencephalon, and Gbx2 is expressed in the rhombencephalon around stage 10. Loss-of-function studies of these genes in mice have revealed that Otx2 is indispensable for the development of the anterior brain segment, and that Gbx2 is required for the development of the isthmus. We carried out gain-of-function(More)
BACKGROUND Leucine-rich repeats (LRRs) are highly versatile and evolvable protein-ligand interaction motifs found in a large number of proteins with diverse functions, including innate immunity and nervous system development. Here we catalogue all of the extracellular LRR (eLRR) proteins in worms, flies, mice and humans. We use convergent evidence from(More)
The forebrain cholinergic system consists of the cholinergic interneurons of the striatum and projection neurons that are distributed in loosely defined groups along the basal forebrain. Fore-brain cholinergic neurons (FCNs) are born between E11 and E15, begin to express cholinergic markers at late embryonic stages, invade their projection target areas(More)
• Handle sections in RNase-free conditions until hybridization is completed.  Wear gloves during operation. Change them when they become dirty.  Use DEPC-treated solution or reagents dissolved in DEPC-treated or nuclease-free water.  Use new clean plasticware (pipettes, etc.). Keep them clean during operation; put them back in the case after operation,(More)
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