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PURPOSE To isolate precursors of human corneal endothelial cells (HCECs) in vitro. METHODS HCECs were subjected to a sphere-forming assay in which spheres floated in serum-free medium containing growth factors. To promote differentiation, the isolated sphere colonies were plated in dishes coated with poly-L-lysine (PLL)/laminin or fetal bovine endothelium(More)
Human corneal endothelial cells (HCECs) have a limited proliferative capacity. Descemet stripping with automated endothelial keratoplasty (DSAEK) has become the preferred method for the treatment of corneal endothelial deficiency, but it requires a donor cornea. To overcome the shortage of donor corneas, transplantation of cultured HCEC sheets has been(More)
BACKGROUND This study investigated whether soluble intercellular adhesion molecule-1 (sICAM-1) has a role in the pathogenesis of macular edema associated with branch retinal vein occlusion (BRVO) together with vascular endothelial growth factor (VEGF). METHODS A retrospective case control study was performed in 22 patients with BRVO and macular edema, as(More)
PURPOSE To evaluate the function of cultured human corneal endothelial cells (HCECs) in vivo and the feasibility of HCEC transplantation with a collagen sheet as the substitute carrier of HCECs. METHODS Adult human donor cornea derived from cultured HCECs was labeled with the fluorescent tracker DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine(More)
PURPOSE To demonstrate the presence of corneal stromal precursors that express neural markers in vitro. METHODS To isolate sphere-forming cells, human corneal stromal cells were subjected to a reaggregation-free neurosphere assay in medium containing methylcellulose gel matrix. To promote differentiation, the isolated sphere colonies were plated in wells(More)
PURPOSE Human corneal endothelial cells (HCECs) are considered to be nonreplicative in vivo; however, isolated HCECs can be cultured and grown successfully, indicating that they retain proliferative capacity. This capacity to replicate tends to decrease with donor age. Cyclin-dependent kinase inhibitors (CKIs) are important negative regulators of the cell(More)
PURPOSE To compare replication competence and senescence in human corneal endothelial cells (HCECs) between the central and peripheral areas and between younger and older donors. METHODS Human corneas were obtained from the eye bank and separated into two groups: young (younger than 30 years) and old (older than 50 years). Corneas were cut in quarters and(More)
PURPOSE To isolate precursor cells derived from rabbit corneal endothelium (CE) and to use them for the treatment of CE deficiency in a rabbit model. METHODS A sphere-forming assay was performed to isolate precursor cells from rabbit CE. Immunocytochemistry was used to examine marker expressions of neural and mesenchymal cells in the sphere colonies and(More)
PURPOSE To establish a method for the mass production of human corneal endothelium (HCE) precursors and the therapeutic application of these cells in a rabbit CE-deficiency model. METHODS A sphere-forming assay was performed to produce precursors from cultured HCE. Various marker expressions were examined in the sphere colonies, and their progenies by(More)
PURPOSE To evaluate the feasibility of autologous transplantation in a rabbit model of conjunctival epithelial cells cultured on amniotic membrane for ocular surface reconstruction. METHODS Limbal stem cell deficiency was induced in the right eyes of 30 rabbits. This was done by performing a lamellar keratectomy of the entire cornea and a complete removal(More)