Tatsuro Takahata

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Retroviral reverse transcription is accomplished by sequential strand-transfers of partial cDNA intermediates copied from viral genomic RNA. Here, we revealed an unprecedented role of 5'-end guanosine (G) of HIV-1 genomic RNA for reverse transcription. Based on current consensus for HIV-1 transcription initiation site, HIV-1 transcripts possess a single G(More)
A major problem for analysis of bio-nanoparticles such as the influenza viruses (size is about 100 nm) is that sample concentration is low. We developed manipulation of the single virus using optical tweezers supported by dielectrophoretic concentration of the viruses in a microfluidic chip. The microfluidic chip made of poly (dimethyl siloxane) (PDMS) is(More)
We developed photo-induced selective injection of nanotool into a specific cell for intracellular measurement in a microfluidic chip. We used membrane fusion of the liposome for invasive cell injection of the nanotool. The nanotool was modified by photo-responsive chemical for pH control and fluorescent dye for environment measurement. The nanotool was(More)
Nonenzymatic roles for HIV-1 integrase (IN) at steps prior to the enzymatic integration step have been reported. To obtain structural and functional insights into the nonenzymatic roles of IN, we performed genetic analyses of HIV-1 IN, focusing on a highly conserved Tyr15 in the N-terminal domain (NTD), which has previously been shown to regulate an(More)
We developed manipulation of single virus using optical tweezers and dielectrophoretic (DEP) force in a microfluidic chip. We used a microfluidic chip made of poly(dimethylsiloxane) (PDMS). The chip has independent virus chamber and cell chamber to make quantitative analysis of the functions of influenza virus before and after infection to a cell. DEP force(More)
We developed Optical pH regulation using functional nanotool impregnated with photo-responsive chemical for selective cell injection of nanotool. The nanotool was modified by fluorescent dye for intracellular measurement. The nanotool was included in the fusogenic liposome. Membrane fusion of the liposome to the cell membrane was used for invasive cell(More)
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