Tatsuro Takahata

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A major problem for analysis of bio-nanoparticles such as the influenza viruses (size is about 100 nm) is that sample concentration is low. We developed manipulation of the single virus using optical tweezers supported by dielectrophoretic concentration of the viruses in a microfluidic chip. The microfluidic chip made of poly (dimethyl siloxane) (PDMS) is(More)
Retroviral reverse transcription is accomplished by sequential strand-transfers of partial cDNA intermediates copied from viral genomic RNA. Here, we revealed an unprecedented role of 5'-end guanosine (G) of HIV-1 genomic RNA for reverse transcription. Based on current consensus for HIV-1 transcription initiation site, HIV-1 transcripts possess a single G(More)
We developed manipulation of single virus using optical tweezers and dielectrophoretic (DEP) force in a microfluid-ic chip. We used a microfluidic chip made of poly(dimethylsiloxane) (PDMS). The chip has independent virus chamber and cell chamber to make quantitative analysis of the functions of influenza virus before and after infection to a cell. DEP(More)
We developed Optical pH regulation using functional nanotool impregnated with photo-responsive chemical for selective cell injection of nanotool. The nanotool was modified by fluorescent dye for intracellular measurement. The nanotool was included in the fusogenic liposome. Membrane fusion of the liposome to the cell membrane was used for invasive cell(More)
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