Tatsuhiko Shimizu

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Our current thinking about the driving force for the movements of intracellular particles or chromosomes has been dominated by our knowledge ofthe two most common con-tractile systems, those based upon actin-myosin interactions or upon microtubule-dynein interactions. Abundant quantities of material in well-ordered arrays have allowed considerable progress(More)
We previously reported the chemical synthesis of oligonucleotides containing thymine glycol, a major form of oxidative DNA damage. In the preparation of the phosphoramidite building block, the predominant product of the osmium tetroxide oxidation of protected thymidine was (5R,6S)-thymidine glycol. To obtain the building block of the other isomer,(More)
We have developed a new model system for studying spindle elongation in vitro using the pennate, marine diatom Cylindrotheca fusiformis. C. fusiformis can be grown in bulk to high densities while in log phase growth and synchronized by a simple light/dark regime. Isolated spindles can be attained in quantities sufficient for biochemical analysis and spindle(More)
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