Tatiana Safronova

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Biologically active compounds were detected in water blooms on Lake Ladoga for the first time. Screening for cytotoxicity and for trypsin and acetylcholinesterase inhibitors revealed a degree of inhibitory activity by the water bloom biomass, which indicates the presence of cyano-bacterial toxins in Lake Ladoga. High performance liquid chromatography (HPLC)(More)
The effects of exogenous ganglioside GM1 (1 microM) from bovine brain on the morphological state and biochemical parameters (creatine kinase, acetylcholinesterase and adenylate cyclase activities as well as the protein, phospholipid and ganglioside content) have been studied in primary cultures of trypsin-treated dissociated cells of chicken embryonic(More)
BB-creatine phosphokinases (CPK-BB, EC 2.7.3.2) were isolated and purified from the chicken brain, heart and gizzard with specific activities 250-300 IU/mg and equal electrophoretic mobilities. Certain differences in thermal stability, activation energy, Km and optimal temperature of the CPK reaction are shown. The amino acid analysis has also revealed(More)
Studies have been made on interaction between rabbit antiserum to BB creatine kinase from hen gizzard and BB creatine kinases from the brain, heart and gizzard of hen. The degree of interaction was evaluated by the intensity of fluorescence of the immune complexes labeled by a fluorescent dye. It was shown that the intensity of fluorescence of the immune(More)
Contractile activity of the frog bladder induced by electrical stimulation of the IX spinal nerve was shown to have a tubocurarine-resistant component. Two main components of the induced motor response were revealed differing from each other in latency, duration and sensitivity to tubocurarine. One of the components seems to be maintained by the activity of(More)
Different methods for the preparation of antibodies to rabbit FITC-labeled MM creatine kinase are compared. Highly specific fluorescent antibodies have been employed to detect MM creatine kinase in isolated cells and in tissue culture. Prospects for research and clinical diagnostic application of the direct method for the detection of creatine kinase in(More)
Effects of copper laser stimulating doses on biochemical parameters of competent chicken embryo myoblast and neuroblast cultures were studied. It was shown that in both systems biostimulation was characterized by an increase of water-extractable protein level and a considerable rise of total and specific creatine kinase activity. The authors believe that(More)
Some properties of creatine kinase were investigated in a two-enzyme system, myosin ATPase-creatine kinase. A rise in the creatine activity in the presence of native myosin was demonstrated. The addition of proteolytic fragments of myosin caused a similar effect. Thermostability of creatine kinase in the presence of native myosin increased. Blocking of(More)
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