Tatiana Coelho-Sampaio

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Regeneration of spinal cord injury (SCI) is a major topic of biomedical research. Laminin is an extracellular matrix protein implicated in neural development and regeneration, but despite that, there are no reports of exogenous laminin contributing to improve the outcome of experimental SCI. Here we investigated whether a biomimetic polymer of laminin(More)
In the developing nervous system migrating neurons and growing axons are guided by diffusible and/or substrate-bound cues, such as extracellular matrix-associated laminin. In a previous work we demonstrated that laminin molecules could self-assemble in two different manners, giving rise to matrices that could favor either neuritogenesis or proliferation of(More)
Cell therapy is a promising strategy to pursue the unmet need for treatment of spinal cord injury (SCI). Although several studies have shown that adult mesenchymal cells contribute to improve the outcomes of SCI, a description of the pro-regenerative events triggered by these cells is still lacking. Here we investigated the regenerative properties of human(More)
Laminin, a major component of basement membranes, can self-assemble in vitro into a typical mesh-like structure, according to a mass-action-driven process. Previously, we showed that pH acidification dramatically increased the efficiency of laminin self-assembly, practically abolishing the necessity for a minimal protein concentration. Here we have(More)
Polylaminin (polyLM) is a flat biomimetic polymer of laminin capable of promoting axonal growth both in vitro and in vivo. It is assembled in a cell-free system when laminin 111 is incubated in acidic pH, whereas incubation in neutral buffer leads to the formation of bulky and irregular polymers (LM). In the present work, we compared the behaviors of cells(More)
Polylaminin (polyLM) is a non-covalent acid-induced nano- and micro-structured polymer of the protein laminin displaying distinguished biological properties. Polylaminin stimulates neuritogenesis beyond the levels achieved by ordinary laminin and has been shown to promote axonal regeneration in animal models of spinal cord injury. Here we used confocal(More)
Tumor growth depends on its capacity to induce formation of new blood vessels [1]. Endostatin, a 20-kDa C-terminal fragment of collagen XVIII, is a potent antiangiogenic agent, fully inhibiting tumor growth in mouse models [2]. Since 2000, endostatin has been tested in patients as an experimental therapeutic agent. Results of clinical trials show that tumor(More)
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