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Two high-resolution structures have been obtained for dihydrofolate reductase from the hyperthermophilic bacterium Thermotoga maritima in its unliganded state, and in its ternary complex with the cofactor NADPH and the inhibitor, methotrexate. While the overall fold of the hyperthermophilic enzyme is closely similar to monomeric mesophilic dihydrofolate(More)
BACKGROUND Dihydroneopterin triphosphate (H2NTP) is the central substrate in the biosynthesis of folate and tetrahydrobiopterin. Folate serves as a cofactor in amino acid and purine biosynthesis and tetrahydrobiopterin is used as a cofactor in amino acid hydroxylation and nitric oxide synthesis. In bacteria, H2NTP enters the folate biosynthetic pathway(More)
The enzyme 6-pyruvoyl tetrahydropterin synthase (PTPS) catalyses the second step in the de novo biosynthesis of tetrahydrobiopterin, the conversion of dihydroneopterin triphosphate to 6-pyruvoyl tetrahydropterin. The Zn and Mg-dependent reaction includes a triphosphate elimination, a stereospecific reduction of the N5-C6 double bond and the oxidation of(More)
Today, enterprises operate a large number of applications providing critical support to the business. These applications form, when taken together, the application landscape, which can be seen as an important asset, providing essential support to business processes, but sometimes also acting as a limiting factor. Important quality attributes depend not only(More)
Workflow automation was first applied for structured processes, for processes which were determined at design time. Decades later case management emerged, workflows which were determined at runtime. Decisions in case management are made by human actors. Case management processing is very labor intensive. How could case management be transferred to a(More)
Research about process instance migration covers mainly changes in process models during the process evolution and their effects on the same runtime environment. But what if the runtime environment - a legacy Business Process Execution (BPE) platform - had to be replaced with a new solution? Several migration aspects must be taken into account. (1) Process(More)
  • G Bader, S Schiffmann, +7 authors T Lemm
  • Journal of molecular biology
  • 2001
Tetrahydrobiopterin, the cofactor required for hydroxylation of aromatic amino acids regulates its own synthesis in mammals through feedback inhibition of GTP cyclohydrolase I. This mechanism is mediated by a regulatory subunit called GTP cyclohydrolase I feedback regulatory protein (GFRP). The 2.6 A resolution crystal structure of rat GFRP shows that the(More)
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