Tamara I. Lyskova

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Brain ischemia is accompanied by lowering of intra- and extracellular pH. Stroke often leads to irreversible damage of synaptic transmission by unknown mechanism. We investigated an influence of lowering of pHi and pHo on free radical formation in synaptosomes. Three models of acidosis were used: (1) pHo 6.0 corresponding to pHi decrease down to 6.04; (2)(More)
The influence of acidosis on the transmembrane potential, sodium pump and membranous systems of calcium transport was studied on isolated presynaptic nerve terminals (synaptosomes) from rat brain. It is established that acidic shift causes a decrease of membrane potential, a large inhibition of the sodium pump (by three times at pH 6.0). All the systems(More)
Effect of neurotoxins veratrine (100 micrograms/ml) and tetrodotoxin (1 microM) on the binding of 3H-ouabain (10(-8) M) with Na,K-ATPase of intact synaptosomes and isolated synaptic membranes was studied. The persistent opening of sodium channels in synaptosomes by veratrine results in an increase of specific binding of the labeled ligand by 20%. A similar(More)
Below 37 degrees the mobility of lipids in rat brain synaptic membranes evaluated by the spin probe method is higher in old (24-26 months) than in young (5-6 months) animals. The number of ouabain receptors in membranes increases with age, while maximum rate and Km values are not changed. The relation between structural state of membrane and properties of(More)
In old rats in contrast to young and mature ones the treatment of synaptic membranes with SH-reagent N-ethylmaleimide, cholinergic agonist carbamylcholine or both of them decreases the specific binding of labeled muscarinic antagonist 3H-quinuclidinylbenzylate. The age related change of receptor conformation with exposure of thiol groups responsible for(More)
Serotonin (10(-6)-10(-3) M) stimulates Na,K-ATPase in the rat brain cortex homogenate with a maximal effect at 10(-4) M. Deseril (10(-5) M), antagonist of serotonin receptors, removes the stimulating effect. Deseril has no influence on noradrenaline-induced ATPase activation; the alpha-adrenergic blocker phentolamine does not affect serotonin activation.(More)
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