Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental… (More)
Insulin stimulates glucose uptake into skeletal muscle tissue mainly through the translocation of glucose transporter 4 (GLUT4) to the plasma membrane. The precise mechanism involved in this process is presently unknown. In the cascade of events leading to insulin-induced glucose transport, insulin activates specific protein kinase C (PKC) isoforms. In this… (More)
In skin, the distribution of integrins is compartmentalized. Whereas the aa IJJ integrin complex is polarized to the basal portion of proliferating cells in the basal layer juxtaposed to the basement membrane, 3II 1 integrin receptors are localized on the cell surface surrounding basal and suprabasal cells, suggesting fii integrins mediate both cell-matrix… (More)
Impaired wound healing of skin is one of the most serious complications of diabetes. However, the pathogenesis of this process is not known, and it is unclear whether impaired insulin signaling could directly affect skin physiology. To elucidate the role of insulin in skin, we studied skin insulin receptor (IR) null mice. The morphology of the skin of… (More)
2752 Overexpression of the Focal Adhesion Kinase (pl25'@â€ •) in Inva sive Human Tumors.
Retinoic acid (RA) was topically applied to the skin of Sencar mice during the promotion phase of specific tumor induction protocols that produce papillomas at low (12-iMetradecanoylphorbol-13-acetate pro moted, TPA) or high (mezerein-promoted) risk for premalignant progres sion and malignant conversion. RA consistently reduced the yield of papillomas and… (More)
We present a system for predicting protein-protein modifications, and demonstrate its usefulness in the field of signal transduction research. Signal transduction is one of the most important areas of investigation in biological research. One of the major mechanisms frequently employed by cells to regulate signal transduction processes involves protein… (More)