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BACKGROUND Optical coherence tomography (OCT) is a depth-resolved, noninvasive, non-destructive imaging modality, the use of which has yet to be fully realized in developmental biology. METHODS AND RESULTS We visualized embryonic chick hearts at looping stages using an OCT system with a 22 micro m axial and 27 micro m lateral resolution and an acquisition(More)
BACKGROUND Congenital conotruncal malformations frequently involve dextroposed aorta. The pathogenesis of dextroposed aorta is not known but is thought to be due to abnormal looping and/or wedging of the outflow tract during early heart development. We examined the stage of cardiac looping in an experimental model of dextroposed aorta to determine the(More)
Previous work demonstrated the power of three-dimensional (3D) magnetic resonance microscopy (MRM) to follow complicated morphologic development in the embryonic cardiovascular system. In this study we describe a new dual-contrast method for specimen preparation that combines perfusion fixation and immersion in fixative with macro- and small molecular(More)
Cardiac neural crest ablation results in primary myocardial dysfunction and failure of the secondary heart field to add the definitive myocardium to the cardiac outflow tract. The current study was undertaken to understand the changes in myocardial characteristics in the heart tube, including volume, proliferation, and cell size when the myocardium from the(More)
C ardiac neural crest (CNC) ablation in embryonic chicks leads to conotruncal anomalies of the heart as a result of altered cardiac looping. Altered looping results from failure of the myocardium from the secondary heart field to be added to the outflow tract. Various imaging techniques have been applied to visualize embryonic heart development. However,(More)
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