Takuya Wada

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The plasma membrane acts as the primary interface between the cellular cytoplasm and the extracellular environment. To investigate the function of the plasma membrane in response to flooding stress, plasma membrane was purified from root and hypocotyl of soybean seedlings using an aqueous two-phase partitioning method. Purified plasma membrane proteins with(More)
A knowledge acquisition method Ripple Down Rules (RDR) can directly acquire and encode knowledge from human experts. It is an incremental acquisition method and each new piece of knowledge is added as an exception to the existing knowledge base. Past researches on RDR method assume that the problem domain is stable. This is not the case in reality,(More)
The " Ripple Down Rules (RDR) " Method is a promising approach to directly acquiring and encoding knowledge from human experts. It requires data to be supplied incrementally to the knowledge base being constructed, each new piece of knowledge being added as an exception to the existing knowledge base. Because of this patching principle, the knowledge(More)
\Ripple Down Rules (RDR)" Method is one of the promising approaches to directly acquire and encode knowledge from human experts. It requires data to be supplied incrementally to the knowledge-base being constructed and new piece of knowledge is added as an exception to the existing knowledge. Because of this patching principle, the knowledge acquired(More)
\Ripple Down Rules (RDR)" Method is one of the promising approaches to directly acquire and encode knowledge from human experts. It requires data to be supplied incrementally to the knowledge-base being constructed and new piece of knowledge is added as an exception to the existing knowledge. Because of this patching principle, the knowledge acquired(More)
" Ripple Down Rules (RDR) " Method is one of the promising approaches to directly acquire and encode knowledge from human experts. It requires data to be supplied incrementally to the knowledge-base being constructed and new piece of knowledge is added as an exception to the existing knowledge-base. Because of this patching principle, the knowledge acquired(More)