Takeshi Karino

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To elucidate the mechanisms of localization of atherosclerotic lesions in man, the effects of various physical and hemodynamic factors on transport of atherogenic low-density lipoproteins (LDL) from flowing blood to the wall of an artery with a multiple bend were studied theoretically by means of a computer simulation under the conditions of a steady flow.(More)
  • T Karino
  • International angiology : a journal of the…
  • 1986
A novel technique to prepare isolated transparent natural blood vessels was developed and used to investigate the connection between blood flow and the localization of vascular diseases in man. Transparent segments of arteries and veins were prepared from dogs and humans postmortem, and the flow patterns in various regions of the circulation were studied in(More)
To clarify the signaling pathways of oxidative stress-induced apoptosis in bovine aortic endothelial cells (BAEC), we treated cells with 1 mM H2O2 and investigated the roles of protein kinase C delta (PKC delta) and Ca2+ in the accumulation of p53 associated with apoptosis. The treatment of cells with H2O2 caused the accumulation of p53, which was inhibited(More)
To examine the involvement of p38 mitogen-activated protein kinase (p38 MAPK) and extracellular signal-regulated kinase (ERK) in the oxidative stress-induced increase of permeability in endothelial cells, the effects of a p38 MAPK inhibitor (SB203580) and ERK inhibitor (PD90859) on the H2O2-induced increase of permeability in bovine pulmonary artery(More)
The viscosity of blood obtained by using a rotational viscometer decreases with the time elapsed from the beginning of measurement until it reaches a constant value determined by the magnitude of shear rate. It is not possible to obtain an initial value of viscosity at time t = 0 that is considered to exhibit an intrinsic property of the fluid by this(More)
To elucidate the mechanisms of localized genesis and development of atherosclerosis and anastomotic intimal hyperplasia in man, a coculture of bovine aortic endothelial cells (ECs) and smooth muscle cells (SMCs) was prepared, and the effects of a shear flow on the uptake of lipoproteins by the cells was studied by incubating the EC–SMC coculture as well as(More)
OBJECT The authors conducted a study to elucidate the relationship between the flow patterns and the formation of aneurysms at the bifurcation of the basilar artery (BA). METHODS Six isolated, transparent vertebrobasilar arterial systems were prepared from humans postmortem, and flow patterns and velocity distributions were studied in detail using flow(More)
We have constructed an in vitro arterial wall model by coculturing bovine arterial endothelial cells (ECs) and smooth muscle cells (SMCs). When ECs were seeded directly over SMCs and cocultured in an ordinary culture medium, ECs grew sparsely and did not form a confluent monolayer. Addition of ascorbic acid to the culture medium at concentrations greater(More)
BACKGROUND The aim of this study is to elucidate the relationship between the flow patterns and the preferred sites of the development of atherosclerotic lesions and cerebral aneurysms in the human ICA and MCA. METHODS Five isolated transparent arterial trees containing the ICA and MCA with a sufficient length of the carotid siphon were prepared from(More)