Takeo Karakida

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Even during the secretory stage of amelogenesis, enamel crystals thicken as amelogenins (the major protein component) decrease. To explain this phenomenon, we propose a model for amelogenin structure and function based upon the hypothesis that amelogenin forms micelles. Solubility and hydrophobicity analyses suggest that all but the hydrophilic amelogenin(More)
Periodontal ligament (PDL) is a thin fibrous connective tissue located between two mineralized tissues, alveolar bone and cementum, which maintains a constant width physiologically. The mechanisms by which PDL resists mineralization are not well understood. Twist is a basic helix loop helix protein that plays a central role in regulation of early(More)
All-trans retinoic acid and bone morphogenetic protein 2 (BMP2) synergistically induced an alkaline phosphatase (ALP) activity, one of the osteoblastic differentiation markers, and promoted the extracellular matrix calcification in a myoblastic C2C12 cell culture system. The induced ALP mRNA was not suppressed in the presence of a protein synthesis(More)
We found previously that in in vitro tube form preparations of isolated intestine of streptozotocin (STZ) diabetic rats, frequency of spontaneous intraluminal pressure waves was significantly reduced in duodenum when compared with normal controls. In order to elucidate further the diabetic intestinal disorders, we examined the frequency and amplitude of(More)
INTRODUCTION Targeting joint destruction induced by osteoclasts (OCs) is critical for management of patients with rheumatoid arthritis (RA). Since phosphoinositide 3-kinase (PI3-K) plays a critical role in osteoclastogenesis and bone resorption, we examined the effects of ZSTK474, a novel phosphoinositide 3-kinase (PI3-K)-specific inhibitor, on murine OCs(More)
The spontaneous motor activity of the isolated segments of the gut of streptozotocin-diabetic rats was studied in vitro. In normal preparations, the frequency of intraluminal pressure changes was little influenced by raising the intraluminal pressure. In contrast, in diabetic preparations, the frequency tended to increase with a rise of the intraluminal(More)
Substance P and somatostatin contents were measured in the gastrointestinal tract of streptozotocin diabetic rats, 1 month after streptozotocin administration (60 mg/kg), and of age-matched controls with radioimmunoassay. Substance P and somatostatin contents were statistically increased in the extrafundus of the diabetic stomach, but not in the diabetic(More)
In order to characterize the diabetic gastrointestinal disorders such as constipation, the motor activity and the compliance of the caecum of streptozotocin diabetic rats were studied in vitro. The time course of enlargement of the caecum was also examined. Significant enlargement of the caecum was noticed in diabetic rats, 10 days after streptozotocin(More)
We found a potent aromatase inhibitor through the screening of agents for estrogen-dependent breast cancer. SEF19 (2-(imidazol-1-yl)-4,6-dimorphorino-1,3,5-triazine) decreased 50% of human placental aromatase activity in vitro at the concentration of 5.3 nM. In order to clarify the selectivity of SEF19 for enzyme inhibition, we determined the effect of(More)
Linear regressions were calculated between blood pressure and heart rate changes during the stimulation of peripheral cut end of cervical sympathetic and vagus nerve in the streptozotocin diabetic rats (65 mg/kg) and age-matched controls. Slopes of blood pressure (mmHg, abscissa)-heart rate (beat/minute, ordinate) relationships of diabetic rats (9.87 +/-(More)