Takayuki Anazawa

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We have devised a simple method for measuring tension development of single myofibrils by micromanipulation with a pair of glass micro-needles. The tension was estimated from the deflection of a flexible needle under an inverted phase-contrast microscope equipped with an image processor, so that the tension development is always accompanied by the(More)
PURPOSE To determine the feasibility, safety and histological change of preoperative endoscopic ultrasound-guided fine-needle injection (PEU-FNI) of immature DCs (iDCs) with OK-432 in pancreatic cancer patients. METHODS Nine patients enrolled in the trial (DC group) and were compared with 15 patients operated on without iDC injection (non-DC group).(More)
Islet autotransplantation (IAT) is used to preserve as much insulin-secretory capacity as possible in patients undergoing total pancreatectomy for painful chronic pancreatitis. The enzyme used to dissociate the pancreas is a critical determinant of islet yield, which is correlated with posttransplant function. Here, we present our experience with IAT(More)
Several years ago, we found a new chemical condition for the spontaneous oscillatory contraction of glycerinated skeletal muscle and named it "SPOC". The condition was such that MgATP coexists with its hydrolytic products, MgADP and inorganic phosphate (Pi). Micromolar concentrations of free Ca2+ were not necessarily required for this oscillation. Here, we(More)
BACKGROUND The optimal enzyme blend that maximizes human islet yield for transplantation remains to be determined. In this study, we evaluated eight different enzyme combinations (ECs) in an attempt to improve islet yield. The ECs consisted of purified, intact or truncated class 1 (C1) and class 2 (C2) collagenases from Clostridium histolyticum (Ch), and(More)
BACKGROUND Islet transplantation is a promising treatment for type 1 diabetes. Due to a shortage of suitable human pancreata, high cost, and the large dose of islets presently required for long-term diabetes reversal; it is important to maximize viable islet yield. Traditional methods of pancreas preservation have been identified as suboptimal due to(More)
The aim of the study was to evaluate preoperative variables predictive of lethal morbidities in critically ill surgical patients at a national level.There is no report of risk stratification for morbidities associated with mortality in critically ill patients with acute diffuse peritonitis (ADP).We examined data from 16,930 patients operated during 2011 and(More)
Islet transplantation is emerging as a promising treatment for patients with type 1 diabetes. It is important to maximize viable islet yield for each organ due to scarcity of suitable human donor pancreata, high cost, and the large dose of islets required for insulin independence. However, organ transport for 8 hours using the two-layer method (TLM)(More)
Skeletal myofibrils spontaneously oscillate under a condition where ATP, ADP, and Pi coexist and the concentration of free Ca2+ is less than about 1 microM. Although this oscillation phenomenon called SPOC is apparently simple, the molecular mechanism seems to be complex. The SPOC condition and the space-time pattern of SPOC wave suggest that the dynamics(More)
BACKGROUND In the absence of a reliable islet potency assay, nude mice (NM) transplantation is the criterion standard to assess islet quality for clinical transplantation. There are factors other than islet quality that affect the transplant outcome. METHODS Here, we analyzed the transplant outcomes in 335 NM receiving islets from human (n=103), porcine(More)