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Class I polyhydroxyalkanoate (PHA) synthase from Ralstonia eutropha (PhaCRe) was engineered so as to acquire an unusual lactate (LA)-polymerizing activity. To achieve this, the site-directed saturation mutagenesis of PhaCRe was conducted at position 510, which corresponds to position 481 in the initially discovered class II LA-polymerizing PHA synthase(More)
The primary structure of HLA-B51 and HLA-Bw52 suggested that HLA-B51 was derived from HLA-Bw52 by the combination of a genetic exchange with HLA-B8 and a point mutation. To investigate the evolution of the HLA-B5 cross reactive group, the HLA-B35 gene was cloned and the primary structure was determined. HLA-B35 is identical to HLA-Bw58 except in the alpha 1(More)
Highly active mutant of NADPH-dependent acetoacetyl-CoA reductase (PhaB) was expressed in Nicotiana tabacum cv. Bright Yellow-2 cultured cells to produce poly(3-hydroxybutyrate) [P(3HB)]. The mutated PhaB increased P(3HB) content by three-fold over the control, indicating that the mutant was a versatile tool for P(3HB) production. Additionally, the(More)
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