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Reduced Genome of the Thioautotrophic Intracellular Symbiont in a Deep-Sea Clam, Calyptogena okutanii
TLDR
The complete genome sequence of the symbiont of Calyptogena okutanii is presented, which is the smallest reported genome in autotrophic bacteria and encodes 939 protein-coding genes, including those for thioautotrophy and for the syntheses of almost all amino acids and various cofactors. Expand
Crystal structures of the group II chaperonin from Thermococcus strain KS-1: steric hindrance by the substituted amino acid, and inter-subunit rearrangement between two crystal forms.
TLDR
It is concluded that this mutant chaperonin with G65C substitution is deprived of the smooth conformational change in the refolding-reaction cycle and obtained a new form of crystal with a distinct space group at a lower concentration of sulfate ion in the presence of nucleotide. Expand
Parasphingopyxis lamellibrachiae gen. nov., sp. nov., isolated from a marine annelid worm.
TLDR
The novel strain isolated from the trophosome of a tubeworm in Kagoshima Bay, Japan, represents a novel species of a new genus in the family Sphingomonadaceae, for which the name Parasphingopyxis lamellibrachiae gen. nov., sp. Expand
The photoperiodic clock in Chymomyza costata
TLDR
Results may suggest that the circadian oscillator involved in the photoperiodic time measurement in this species damps out after 24 h, as indicated by the small peak of diapause incidence observed when T was 24 h. Expand
Archaeal group II chaperonin mediates protein folding in the cis-cavity without a detachable GroES-like co-chaperonin.
TLDR
The present data suggest that AMP-PNP induced one of two chaperonin rings (cis-ring) to close and induced protein refolding in this ring, and that the other ring could capture the unfolded GFP which was refolded by adding ATP. Expand
Molecular Evidence that Phylogenetically Diverged Ciliates Are Active in Microbial Mats of Deep‐Sea Cold‐Seep Sediment
TLDR
Environmental RNA and DNA from microbial mats of cold‐seep sediment in Sagami Bay, Japan, are isolated and retrieved eukaryotic small‐subunit ribosomal RNA sequences with polymerase chain reaction methods followed by clone library construction, suggesting that ciliates are active in the environment. Expand
Heterogeneous composition of key metabolic gene clusters in a vent mussel symbiont population
TLDR
It is reported that a single-ribotype endosymbiont population in an individual of the host vent mussel, Bathymodiolus septemdierum has heterogeneous genomes with regard to the composition of key metabolic gene clusters for hydrogen oxidation and nitrate reduction. Expand
RECENT HOST RANGE EXPANSION OF CANINE DISTEMPER VIRUS AND VARIATION IN ITS RECEPTOR, THE SIGNALING LYMPHOCYTE ACTIVATION MOLECULE, IN CARNIVORES
TLDR
To understand the recent host range expansion of canine distemper virus (CDV) in carnivores, nucleotide sequences of SLAMs of various carnivores were determined and three-dimensional homology SLAM models were generated. Expand
Expression of genes for sulfur oxidation in the intracellular chemoautotrophic symbiont of the deep-sea bivalve Calyptogena okutanii
TLDR
The result of the RT-PCR showed that genes encoding key enzymes catalyzing sulfur oxidation were all equally expressed in the Vok under three different environmental conditions, indicating that all sulfur oxidation pathways function simultaneously to support intracellular symbiotic life. Expand
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