Takako Yamaguchi

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  • T Yamaguchi
  • The Bulletin of Tokyo Medical and Dental…
  • 1986
Extracellular potassium and calcium ion concentration, field potential (Fp), local EEG and cortical impedance were measured continuously in the cortex and hippocampus of the pentobarbital anesthetized gerbils subjected to 5-minute ischemia produced by bilateral carotid occlusion. Extracellular potassium ([K+]e) and extracellular calcium ([Ca++]e) showed a(More)
Glycobiology pertains to the study of carbohydrate sugar chains, or glycans, in a particular cell or organism. Many computational approaches have been proposed for analyzing these complex glycan structures, which are chains of monosaccharides. The monosaccharides are linked to one another by glycosidic bonds, which can take on a variety of comformations,(More)
Methods for the fluorophotometric determination of hydrogen peroxide (H(2)O(2)) and other reactive oxygen species (ROS) were proposed by using the fluorescence reaction between H(2)O(2) or other ROS and fluorescein hydrazide (FH). In the determination of H(2)O(2), the calibration curve exhibited linearity over the H(2)O(2) concentration range of 2.1-460 ng(More)
A simple and highly sensitive spectrophotometric method for the determination of uric acid (UA) was established based on fading of the o-hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethylammonium complex. In the determination of UA, Beer's law is obeyed in the range of 0.01-0.20 microg ml(-1), with an effective molar absorptivity at 635 nm, the(More)
A simple and highly sensitive spectrophotometric method for the determination of biologically active thiols based on the fading of eosin-silver(I)-adenine ternary complex was established. In the determination of 6-mercaptopurine (MP), Beer's law was obeyed in the range 0.02-0.30 microg ml(-1), with an effective molar absorptivity at 562 nm and the relative(More)
Spectrophotometric determination of hydrogen peroxide was accomplished with osmium(VIII) and m-carboxyphenylfluorone (MCPF) in the presence of cetyltrimethylammonium chloride (CTAC). In the determination of hydrogen peroxide based on the fading of the color of osmium(VIII)-MCPF complex, Beer's law was obeyed in the range 20-406 ng mL(-1), with an effective(More)
BACKGROUND Aged garlic extract (AGE) has been shown to retard the progression of coronary calcification in patients with coronary artery disease. OBJECTIVE To clarify the mechanism of AGE's action to retard atherosclerosis, we investigated whether AGE suppresses the formation and progression of atherosclerosis in Apolipoprotein E (Apoe)-knockout (ApoE-KO)(More)
A simple and sensitive spectrophotometric method for the determination of quinolone antibiotics was established based on an association complex formation with aluminum(III) and erythrosin. In the determination of ofloxacin as a quinolone antibiotic, Beer's law is obeyed in the range of 0.1 - 3.2 microg ml(-1), with an effective molar absorptivity at 555 nm(More)
OBJECTIVES S-Allylcysteine (SAC) and S-1-propenylcysteine (S1PC) are the characteristic sulfur-containing amino acids in aged garlic extract. In this study, we investigated the effect of SAC and S1PC on intestinal immunoglobulin (Ig)A production to gain insight into the immunomodulatory effect of aged garlic extract. METHODS In vitro study: Mouse splenic(More)
Rhodamine B hydrazide can be used to detect hydroxyl radicals in plant cells. RBH was easily inserted into plant cells without any pretreatment, and specifically reacted with intracellular hydroxyl radicals produced by antimycin A. RBH will be a powerful tool for detecting hydroxyl radicals in plant cells.