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The thermosensitivity of 65 spontaneously active neurons in the paraventricular nucleus (PVN) was investigated by extracellular recording in the rat hypothalamic slice preparation. The firing rate of these cells was comparatively low, ranging from 0.03 to 10.0 (mean 2.46) impulses/s at 37 degrees C, and only a minority showed a phasic firing pattern. Of 65(More)
The site of action for the sleep-promoting effect of prostaglandin (PG) D2 was extensively examined in the brain of adult male rats (n = 231). PGD2 was administered at 100 pmol/0.2 microliter per min for 6 hr (2300-0500 hr) through chronically implanted microdialysis probes or infusion cannulae. Among the administrations of PDG2 by dialysis probes (n =(More)
Extracellular recordings were made from 467 anti-dromically identified neurosecretory neurons and 148 non-neurosecretory neurons in the paraventricular nucleus of the hypothalamus of hemispherectomized cats under pentobarbital anesthesia. Stimulation of the dorsal, median, and pontine raphe nuclei excited 31%, 26%, and 12% of neurosecretory neurons tested,(More)
Extracellular recordings were made from 44 spontaneously active neurosecretory and 43 unidentified neurons in the paraventricular nucleus (PVN) of the hypothalamus in urethane-anesthetized rats. Physiologically hypertonic (+30 mOsm/kg, NaCl or mannitol) and hypotonic (-30 mOsm/kg) solutions were applied by pressure through multibarrel micropipettes to the(More)
The effects of gamma-aminobutyric acid (GABA) antagonists on baroreceptor-activated inhibition of neurosecretory cells in the paraventricular nucleus (PVN) were examined in urethane-chloralose anesthetized rats. In 11 neurosecretory cells which were inhibited by baroreceptor activation induced by intravenous application of phenylephrine,(More)
In male rats anesthetized with urethane-chloralose, extracellular recordings were made from tuberoinfundibular (TI) neurons in the paraventricular nucleus (PVN) identified by antidromic stimulation of the median eminence. Activation of baroreceptors induced by intravenous administration of phenylephrine inhibited approximately two-thirds of the 32(More)
Responses of sleep-related neurons in the preoptic area (POA) to stimulation of the locus coeruleus (LC) and the ventrolateral medulla (VLM), components of the reticular activating system, were recorded in the unanesthetized, head-restrained rat. Single-pulse stimulation of the LC and the VLM, respectively, inhibited 50% and 54% of 30 sleep-active neurons(More)
We investigated the postnatal development of L-type Ca2+ current (ICa) in enzymatically isolated adult (AD) and newborn (NB) (1-3-day-old) rabbit ventricular cells using the whole-cell, patch-clamp method. ICa was recorded with Cs(+)-rich pipettes and a Na(+)- and K(+)-free bath solution at 36 degrees C to eliminate other currents. ICa density (obtained by(More)
Prostaglandin D2 (PGD2) was applied by pressure through a multibarrel micropipette to sleep-related neurons in the preoptic and neighboring basal forebrain area in head-restrained unanesthetized rats. During wakefulness, PGD2 excited six of 13 sleep-active neurons, inhibited seven of 13 waking-active neurons, and did not affect the remaining neurons. During(More)
A premature action potential elicited in ventricular muscle during the functional refractory period of a preceding action potential requires an increased stimulus intensity for successful propagation. We measured the cellular basis for these relative decreases in tissue excitability during the recovery phase by performing parallel experiments on rabbit left(More)