Taisuke Matsuda

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Zinc finger nuclease (ZFN) is a powerful tool for genome editing. ZFN-encoding plasmid DNA expression systems have been recently employed for the generation of gene knockout (KO) pigs, although one major limitation of this technology is the use of potentially harmful genome-integrating plasmid DNAs. Here we describe a simple, non-integrating strategy for(More)
BACKGROUND The development and validation of stem cell therapies using induced pluripotent stem (iPS) cells can be optimized through translational research using pigs as large animal models, because pigs have the closest characteristics to humans among non-primate animals. As the recent investigations have been heading for establishment of the human iPS(More)
PURPOSE To investigate the effects of the continuous viewing of horizontal disparities on the pupillary near response by using three-dimensional (3-D) displays. METHODS The pupillary diameter and the horizontal eye position of both eyes were measured during the near response in eight healthy young adults. The measurements were made before and after(More)
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