Taihei Onishi

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OBJECTIVES To assess the structure and extracellular matrix molecule expression of osteogenic cell sheets created via culture in medium with both dexamethasone (Dex) and ascorbic acid phosphate (AscP) compared either Dex or AscP alone. METHODS Osteogenic cell sheets were prepared by culturing rat bone marrow stromal cells in a minimal essential medium(More)
Copper and zinc concentrations, and the nature of the copper- and zinc-binding proteins, were studied using tissues from a Menkes patient who had been given intravenous infusions of cupric acetate. The liver and brain copper contents were lower than in an untreated, non-Menkes control, and the spleen, intestine and kidney showed higher copper concentrations(More)
Menkes fibroblast cultures were established and copper metabolism was investigated. Menkes fibroblasts contained four to six times higher concentrations of copper than control cells. In Menkes cells more than 90% of the intracellular copper was present in cytosol (105,000 x g supernatant fraction); in control fibroblasts the corresponding value was about(More)
― ― S10 ― In early stage of atherosclerosis, infiltration of blood leukocytes especially monocytes across vascular endothelial cells (ECs) in intima is thought to be key events to disease progression. We have analyzed two major junctional proteins, PECAM-1 and VEcadherin in ECs during trans-endothelial migration (TEM) of monocytes in 3 dimensional mult i(More)
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