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Using a mouse embryo cDNA library, we conducted a two-hybrid screening to identify new partners for the small GTPase Rho. One clone obtained by this procedure contained a novel cDNA of 291 base pairs and interacted strongly with RhoA and RhoC, weakly with RhoB, and not at all with Rac1 and Cdc42Hs. Full-length cDNAs were then isolated from a mouse brain(More)
Rho small GTPase regulates cell morphology, adhesion and cytokinesis through the actin cytoskeleton. We have identified a protein, p140mDia, as a downstream effector of Rho. It is a mammalian homolog of Drosophila diaphanous, a protein required for cytokinesis, and belongs to a family of formin-related proteins containing repetitive polyproline stretches.(More)
Using the yeast two hybrid system and overlay assays we identified a putative rholrac effector, citron, which interacts with the GTP-bound forms of rho and rac1, but not with cdc42. Extensive homologies to known proteins were not observed. This 183 kDa protein contains a C6H2 zinc finger, a PH domain, and a long coiled-coil forming region including 4(More)
Within the mechanical engineering discipline, product representation studies have been used to inform engineers on the suitability of their product designs for prospective customers. However, these studies are mainly based in customers' oral responses leading engineers to modify the product design accordingly. In contrast, we consider the eye tracking data(More)
Mitigating human errors is a priority in the design of complex systems, especially through the use of body area networks. This paper describes early developments of a dynamic data driven platform to predict operator error and trigger appropriate intervention before the error happens. Using a two-stage process, data was collected using several sensors (e.g.,(More)
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