Tadeusz Rorat

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Dehydrins (DHNs) are part of a large group of highly hydrophilic proteins known as LEA (Late Embryogenesis Abundant). They were originally identified as group II of the LEA proteins. The distinctive feature of all DHNs is a conserved, lysine-rich 15-amino acid domain, EKKGIMDKIKEKLPG, named the K-segment. It is usually present near the C-terminus. Other(More)
The expression of a gene, designated as DHN10, was analyzed at the protein level in two Solanum species. The DHN10 protein displays some consensus amino acid sequences of dehydrins, termed K- and S-segments. Unlike most dehydrins, both segments occur only in single copies in the DHN10 sequence and the S-segment is at a C-terminal position. Database searches(More)
The expression of a gene, encoding a dehydrin protein designated as DHN24 was analyzed at the protein level in two groups of Solanum species differing in cold acclimation ability. The DHN24 protein displays consensus amino acid sequences of dehydrins, termed K- and S-segments. The S-segment precedes three K-segments, classifying the protein into SK3-type(More)
In order to characterize a β-amylase deficiency in the endosperm of mutant rye lines, homologous cDNA probes were prepared. A rye cDNA library was constructed from a normal line and screened with a barley β-amylase probe. Three partial cDNA clones specific for endosperm β-amylase in rye were isolated and characterized. The largest of these clones was used(More)
This report deals with the effect of kinetin on the greening process, in relation to endogenous free polyamine levels and their metabolism in cucumber cotyledons. The kinetin response on free polyamine levels was found to be accompanied by an increase in free putrescine throughout the greening process. There was no significant difference in spermidine and(More)
The expression of a gene isolated from cDNA differential screening and encoding a lipid transfer protein, designated as SsLTP1, was analysed at the protein level in two groups of Solanum species and lines differing in cold acclimation capacity. Under control conditions, the SsLTP1 was localized in all aerial organs of S. sogarandinum and S. tuberosum(More)
The role of acidic SKn dehydrins in stress tolerance of important crop and model species of the Solanaceae remains unknown. We have previously shown that the acidic SK3 dehydrin DHN24 from Solanum sogarandinum is constitutively expressed and its expression is associated with cold acclimation. Here we found that DHN24 is specifically localized to phloem(More)
Changes in physiological parameters (relative water content (RWC), biomass, water use efficiency (WUE), net photosynthetic yield (PN) and quantum yield of PSII (Fv/Fm)), in proline and sugar content, and expression profile of genes reported to be associated with the barley response to water deficit, including LEA genes, NHX1, Hsdr4, BLT101 and genes(More)
The abundance of a single domain cyclophilin (CyP), designated as SsCyP, was investigated in Solanum sogarandinum and Solanum tuberosum plants during development and in response to various environmental constraints. We show that under control conditions, SsCyP is distributed throughout the plant but in an organ-specific manner. In both Solanum species, the(More)
BACKGROUND AND AIMS Double B-box zinc finger (DBB) proteins are recently identified plant transcription regulators that participate in the response to sodium chloride-induced stress in arabidopsis plants. Little is known regarding their subcellular localization and expression patterns, particularly in relation to other osmotic constraints and the day/night(More)