Tadanori Ohsugi

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A methanolic extract ofCitrus unshiu induces oviposition by females of aCitrus-feeding swallowtail butterfly,Papilio xuthus L. The chemical factors responsible for stimulating oviposition were isolated and characterized as 5-hydroxy-Nω-methyltryptamine, adenosine, vicenin-2, narirutin, hesperidin and rutin. An artificial blend of these six components(More)
Nattokinase (NK) is a strong fibrinolytic enzyme, which is produced in abundance by Bacillus subtilis natto. Although NK is a member of the subtilisin family, it displays different substrate specificity when compared with other subtilisins. The results of molecular simulations predict that hydrogen arrangements around Ser221 at the active site probably(More)
Nattokinase is a single polypeptide chain composed of 275 amino acids (molecular weight 27,724) which displays strong fibrinolytic activity. Moreover, it can activate other fibrinolytic enzymes such as pro-urokinase and tissue plasminogen activator. In the present study, native nattokinase from Bacillus subtilis natto was purified using gel-filtration(More)
s: It was found that when dipicolinic acid is added to the culture solution of Bacillus subtilis natto, the area of fi-brin dissolved by nattokinase (standard fibrin plate) and the amidase activity of nattokinase against Suc-Ala-Ala-Pro-Phe-pNA increased. For example, when manufacturing natto using steamed soybeans, the addition of 10-64 mM of dipicolinic(More)
Natto is a traditional Japanese food made from soybeans fermented by Bacillus subtilis natto. Natto and natto fungi contain many physiologically active substances, including dipicolinic acid, nattokinase, and vitamin K2. We used a rat hindleg perfusion model to show that the novel substances present in natto improve thrombolytic activity in the blood. The(More)
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