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An enhanced system for unnatural amino acid mutagenesis in E. coli.
We report a new vector, pEVOL, for the incorporation of unnatural amino acids into proteins in Escherichia coli using evolved Methanocaldococcus jannaschii aminoacyl-tRNA synthetase(s)Expand
Coordinated Regulation of Accessory Genetic Elements Produces Cyclic Di-Nucleotides for V. cholerae Virulence
The function of the Vibrio 7(th) pandemic island-1 (VSP-1) in cholera pathogenesis has remained obscure. Utilizing chromatin immunoprecipitation sequencing and RNA sequencing to map the regulon ofExpand
Beyond the Canonical 20 Amino Acids: Expanding the Genetic Lexicon*
The ability to genetically encode unnatural amino acids beyond the common 20 has allowed unprecedented control over the chemical structures of recombinantly expressed proteins. OrthogonalExpand
Versatile strategy for controlling the specificity and activity of engineered T cells
Significance Despite the unprecedented antileukemic response demonstrated in recent clinical trials, the inability to control the potent chimeric antigen receptor (CAR)—T-cell activity has resultedExpand
Switch-mediated activation and retargeting of CAR-T cells for B-cell malignancies
Significance Chimeric antigen receptor T (CAR-T) cell therapy has produced promising results in clinical trials but has been challenged by the inability to control engineered cells once infused intoExpand
Redirection of genetically engineered CAR-T cells using bifunctional small molecules.
Chimeric antigen receptor (CAR)-engineered T cells (CAR-Ts) provide a potent antitumor response and have become a promising treatment option for cancer. However, despite their efficacy, CAR-T cellsExpand
Identification of the thiazolyl peptide GE37468 gene cluster from Streptomyces ATCC 55365 and heterologous expression in Streptomyces lividans
  • T. Young, C. Walsh
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • 25 July 2011
Thiazolyl peptides are bacterial secondary metabolites that potently inhibit protein synthesis in Gram-positive bacteria and malarial parasites. Recently, our laboratory and others reported that thisExpand
An anti-B cell maturation antigen bispecific antibody for multiple myeloma.
The development of immunotherapies for multiple myeloma is critical to provide new treatment strategies to combat drug resistance. We report a bispecific antibody against B cell maturation antigenExpand
An evolved aminoacyl-tRNA synthetase with atypical polysubstrate specificity.
We have employed a rapid fluorescence-based screen to assess the polyspecificity of several aminoacyl-tRNA synthetases (aaRSs) against an array of unnatural amino acids. We discovered that aExpand
Design of Switchable Chimeric Antigen Receptor T Cells Targeting Breast Cancer.
Chimeric antigen receptor T (CAR-T) cells have demonstrated promising results against hematological malignancies, but have encountered significant challenges in translation to solid tumors. ToExpand