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Screening for the Preferred Substrate Sequence of Transglutaminase Using a Phage-displayed Peptide Library
The amino acid sequences that demonstrated higher specificity and inhibitory activity in the cross-linking reactions by TGase 2 and Factor XIIIa were identified and confirmed that the sequences were favored for transamidation using modified glutathione S-transferase (GST) for recombinant peptide-GST fusion proteins. Expand
Cysteine sulfinate desulfinase, a NIFS-like protein of Escherichia coli with selenocysteine lyase and cysteine desulfurase activities. Gene cloning, purification, and characterization of a novel
The Escherichia coli genome contains three genes with sequence homology to nifS, and a new enzyme catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L- Cysteine sulfinate desulfinase, and L-selenocystine to produce L-alanine is named. Expand
Crystal Structure of a Homolog of Mammalian Serine Racemase from Schizosaccharomyces pombe*
The crystal-soaking experiment showed that the substrate serine was actually trapped in the active site of the modified enzyme, suggesting that the lysino-d-alanyl residue acts as a catalytic base in the same manner as inherent Lys-57 of the wild-type enzyme. Expand
Cold-Active Serine Alkaline Protease from the Psychrotrophic Bacterium Shewanella Strain Ac10: Gene Cloning and Enzyme Purification and Characterization
The recombinant SapSh (rSapSh) was found to have a molecular weight of about 44,000 and to be highly active in the alkaline region (optimum pH, around 9.0) when azocasein and synthetic peptides were used as substrates, but was far less stable than the subtilisin. Expand
Kinetic and mutational studies of three NifS homologs from Escherichia coli: mechanistic difference between L-cysteine desulfurase and L-selenocysteine lyase reactions.
Three NifS homologs from Escherichia coli, CSD, CsdB, and IscS, that appear to be involved in iron-sulfur cluster formation and/or the biosynthesis of selenophosphate are purified, suggesting the presence of different rate-limiting steps or reaction mechanisms for L-cysteine desulfurization and the degradation of L-selenocysteine. Expand
Cys-328 of IscS and Cys-63 of IscU are the sites of disulfide bridge formation in a covalently bound IscS/IscU complex: Implications for the mechanism of iron-sulfur cluster assembly
  • S. Kato, H. Mihara, +4 authors N. Esaki
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences…
  • 23 April 2002
A mechanism for an early stage of iron-sulfur cluster assembly is proposed: the sulfur transfer from IscS to Isc U is initiated by the attack of Cys-63 of IscU on the Sγ atom of CYS-328 of IsCS that is bound to sulfane sulfur derived from l-cysteine. Expand
cDNA Cloning, Purification, and Characterization of Mouse Liver Selenocysteine Lyase
Reverse transcriptase-polymerase chain reaction and Western blot analyses revealed that mSCL is cytosolic and predominantly exists in the liver, kidney, and testis, where mouse selenophosphate synthetase is also abundant, supporting the view that pyridoxal phosphate-dependent and highly specific tol-selenocysteine functions in cooperation with selenoprotein synthesis. Expand
Synthesis of optically active amino acids from alpha-keto acids with Escherichia coli cells expressing heterologous genes
A simple method for enzymatic synthesis of L and D amino acids from alpha-keto acids with Escherichia coli cells which express heterologous genes is described and optic pure D enantiomers of glutamate and leucine were obtained. Expand
Three-dimensional structure of 4-amino-4-deoxychorismate lyase from Escherichia coli.
The crystal structure of ADCL from Escherichia coli has been solved using MIR phases in combination with density modification and the structure has been refined to an R-factor of 20.6% at 2.2 A resolution. Expand
Conserved Pyridoxal Protein That Regulates Ile and Val Metabolism
It is demonstrated for the first time that YggS controls Ile and Val metabolism by modulating 2-ketobutyrate and CoA availability and fully reversed phenotypes conferred by the yggS mutation. Expand