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Recombinant expression, biochemical characterization and stabilization through proteolysis of an L-glutamate oxidase from Streptomyces sp. X-119-6.
L-glutamate oxidase (LGOX) from Streptomyces sp. X-119-6 is a protein of 150 kDa that has hexamer structure alpha2beta2gamma2. The gene encoding LGOX was cloned and heterologously expressed inExpand
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Purification and characterization of an endo-1,4-β-mannanase from Bacillus subtilis KU-1
Endo-1,4-β-mannanase was purified to homogeneity from the culture supernatant of Bacillus subtilis KU-1 by ammonium sulfate precipitation, DEAE-Toyopearl, phenyl-Sepharose and FPLC Mono Q columnExpand
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Unusual ligand structure in Ni-Fe active center and an additional Mg site in hydrogenase revealed by high resolution X-ray structure analysis.
BACKGROUND The hydrogenase of Desulfovibrio sp. catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3. The Ni-Fe active centerExpand
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Cysteine sulfinate transamination activity of aspartate aminotransferases.
Abstract Aspartate aminotransferases from pig heart cytosol and mitochondria, Escherichia coli B and Pseudomonas striata accepted L-cysteine sulfinate as a good substrate. The mitochondrial isoenzymeExpand
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Role of Walker Motif A of RuvB Protein in Promoting Branch Migration of Holliday Junctions
Escherichia coli RuvB protein, an ATP-dependent hexameric DNA helicase, acts together with RuvA protein to promote branch migration of Holliday junctions during homologous recombination andExpand
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Trends in antimicrobial-drug resistance in Japan.
Multidrug resistance in gram-positive bacteria has become common worldwide. In Japan until recently, gram-negative bacteria such as Pseudomonas aeruginosa, Klebsiella pneumoniae, and SerratiaExpand
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Thiols in oxidative phosphorylation: inhibition and energy-potentiated uncoupling by monothiol and dithiol modifiers.
Three apparently different modifications of submitochondrial particles (SMP) or ATP synthase preparations (complex V) inhibit oxidative phosphorylation and ATP-32Pi exchange activities, all of whichExpand
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Lack of Complex I Activity in Human Cells Carrying a Mutation in MtDNA-encoded ND4 Subunit Is Corrected by theSaccharomyces cerevisiae NADH-Quinone Oxidoreductase (NDI1) Gene*
The gene for the single subunit, rotenone-insensitive, and flavone-sensitive internal NADH-quinone oxidoreductase of Saccharomyces cerevisiae(NDI1) can completely restore the NADH dehydrogenaseExpand
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Purification, molecular cloning, and catalytic activity of Schizosaccharomyces pombe pyridoxal reductase. A possible additional family in the aldo-keto reductase superfamily.
Pyridoxal reductase (PL reductase), which catalyzes reduction of PL by NADPH to form pyridoxine and NADP(+), was purified from Schizosaccharomyces pombe. The purified enzyme was very unstable but wasExpand
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