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Efficient duck hepatitis B virus production by an avian liver tumor cell line
The results substantiate the hypothesis that covalently closed circular DNA is synthesized by the viral reverse transcriptase, and produce sufficient virus with the mutant genome to investigate the role of polymerase in covalent closed circularDNA amplification.
Evidence that two latency-associated transcripts of herpes simplex virus type 1 are nonlinear
The data show that the two major latency-associated transcripts of herpes simplex virus type 1 are nonlinear, and they support an interpretation of stable lariat structures.
Identical V region amino acid sequences and segments of sequences in antibodies of different specificities. Relative contributions of VH and VL genes, minigenes, and complementarity-determining…
It will be important in the future to investigate further conformational effects on antibody structure by using X-ray crystallography, nuclear magnetic resonance spectroscopy, or other methods, to obtain a better understanding of the functions and topography of antibody-combining sites.
Replication of DHBV genomes with mutations at the sites of initiation of minus- and plus-strand DNA synthesis.
Effects of nucleotide changes on the ability of hepatitis delta virus to transcribe, process, and accumulate unit-length, circular RNA
The circular RNA genome of hepatitis delta virus (HDV) can fold into an unbranched rodlike structure. We mutagenized the two ends of this structure and assayed the effects on the ability of the…
Unusual distributions of amino acids in complementarity-determining (hypervariable) segments of heavy and light chains of immunoglobulins and their possible roles in specificity of antibody-combining…
In hepatocytes infected with duck hepatitis B virus, the template for viral RNA synthesis is amplified by an intracellular pathway.
Rapid resolution of duck hepatitis B virus infections occurs after massive hepatocellular involvement
Pekin ducks, congenitally infected with the duck hepatitis B virus, were used to assess age-dependent variations in viremia, percentage of DHBV-infected hepatocytes, and average levels of DNA replication intermediates in the cytoplasm and of covalently closed circular DNA in the nuclei of infected hepatocytes.
Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions.
The primary structures of three human forms of secretory carrier membrane proteins (SCAMPs) have been deduced from full-length clones isolated from a HeLa cell cDNA library and confirmed by a…
Evidence for colocalization and interaction between 37 and 39 kDa isoforms of secretory carrier membrane proteins (SCAMPs).
It is suggested that both SCAMP1 and SCAMP2 may function together in a single protein complex, like other transport vesicle membrane proteins, that apparently include homomultimers.