Cryptic prophages help bacteria cope with adverse environments
It is found that cryptic prophages contribute significantly to resistance to sub-lethal concentrations of quinolone and β-lactam antibiotics primarily through proteins that inhibit cell division (for example, KilR of rac and DicB of Qin).
Indole is an inter-species biofilm signal mediated by SdiA
It is shown here that indole is a non-toxic signal that controls E. coli biofilms by repressing motility, inducing the sensor of the quorum sensing signal autoinducer-1 (SdiA), and influencing acid resistance (e.g., hdeABD, gadABCEX).
The bacterial signal indole increases epithelial-cell tight-junction resistance and attenuates indicators of inflammation
- T. Bansal, R. Alaniz, T. Wood, A. Jayaraman
- BiologyProceedings of the National Academy of Sciences
- 29 December 2009
Exposure to physiologically relevant amounts of indole increased expression of genes involved in strengthening the mucosal barrier and mucin production, which were consistent with an increase in the transepithelial resistance of HCT-8 cells, suggesting that indole could be important in the intestinal epithelial cells response to gastrointestinal tract pathogens.
Autoinducer 2 Controls Biofilm Formation in Escherichia coli through a Novel Motility Quorum-Sensing Regulator (MqsR, B3022)
- A. G. González Barrios, R. Zuo, Y. Hashimoto, Li Yang, W. Bentley, T. Wood
- BiologyJournal of Bacteriology
- 1 January 2006
Results indicate AI-2 induces biofilm formation in E. coli through B3022, which then regulates QseBC and motility; hence, b3022 has been renamed the motility quorum-sensing regulator gene (the mqsR gene).
Gene expression in Escherichia coli biofilms
- D. Ren, L. Bedzyk, S. M. Thomas, R. Ye, T. Wood
- Biology, EngineeringApplied Microbiology and Biotechnology
- 16 January 2004
The present study suggests that biofilm gene expression is strongly associated with environmental conditions and that stress genes are involved in E. coli JM109 biofilm formation.
Differential Effects of Epinephrine, Norepinephrine, and Indole on Escherichia coli O157:H7 Chemotaxis, Colonization, and Gene Expression
- T. Bansal, Derek L. Englert, Jintae Lee, M. Hegde, T. Wood, A. Jayaraman
- Biology, ChemistryInfection and Immunity
- 25 June 2007
Compared to indole, epinephrine and norepinephrine exert divergent effects on EHEC chemotaxis, motility, biofilm formation, gene expression, and colonization of HeLa cells.
Three Dimensional Structure of the MqsR:MqsA Complex: A Novel TA Pair Comprised of a Toxin Homologous to RelE and an Antitoxin with Unique Properties
It is revealed that MqsR:MqsA form a novel toxin:antitoxin (TA) pair and that TA systems, especially the antitoxins, are significantly more diverse than previously recognized and provide new insights into the role of toxins in maintaining the persister state.
Temporal gene-expression in Escherichia coli K-12 biofilms.
Cold-shock protein transcriptional regulators (cspABFGI) appear to be positive biofilm regulators, and deletions in respiratory genes (e.g. gatZABCDR, agaBCY) were highly regulated in biofilms.
Convergence Patterns in Subsocial Insects
In the insects, parental behavior lies at the core of all levels of insect sociality and has arisen independently in at least 13 different orders, and Wilson (172) has identified four environmental "prime movers" that create conditions favorable to the evolution of parental care.
YdgG (TqsA) Controls Biofilm Formation in Escherichia coli K-12 through Autoinducer 2 Transport
YdgG was found to negatively modulate expression of flagellum- and motility-related genes, as well as other known products essential for biofilm formation, including operons for type 1 fimbriae, autotransporter protein Ag43, curli production, colanic acid production, and production of polysaccharide adhesin.