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Production of infectious hepatitis C virus in tissue culture from a cloned viral genome
TLDR
It is shown that the JFH1 genome replicates efficiently and supports secretion of viral particles after transfection into a human hepatoma cell line (Huh7) and provides a powerful tool for studying the viral life cycle and developing antiviral strategies.
Robust hepatitis C virus infection in vitro.
TLDR
A simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture is reported.
The lipid droplet is an important organelle for hepatitis C virus production
TLDR
A novel function of LDs is revealed in the assembly of infectious HCV and a new perspective on how viruses usurp cellular functions is provided.
Interaction of Hepatitis C Virus Nonstructural Protein 5A with Core Protein Is Critical for the Production of Infectious Virus Particles
TLDR
It is demonstrated that NS5A is a prerequisite for HCV particle production as a result of its interaction with the viral capsid protein (core protein), and that the alanine substitutions in the serine cluster suppressed the association of the core protein with viral genome RNA, possibly resulting in the inhibition of nucleocapsid assembly.
Efficient replication of the genotype 2a hepatitis C virus subgenomic replicon.
TLDR
The genotype 2a subgenomic replicon was established in Huh7 cells and replicated efficiently with or without G418 selection in a transient replication assay, providing a powerful new tool for researching HCV.
Human P-selectin glycoprotein ligand-1 is a functional receptor for enterovirus 71
Enterovirus 71 (EV71) is a major causative agent of hand, foot and mouth disease (HFMD), a common febrile disease occurring mainly in young children. Although clinical manifestations of HFMD are
Induction of incomplete autophagic response by hepatitis C virus via the unfolded protein response
TLDR
It is demonstrated by a battery of morphological and biochemical assays that hepatitis C virus induces the accumulation of autophagosomes in cells without enhancing autophagic protein degradation, indicating a positive role of UPR and the incomplete Autophagic response in HCV replication.
Production of infectious genotype 1a hepatitis C virus (Hutchinson strain) in cultured human hepatoma cells
TLDR
Production of infectious genotype 1a HCV in cells transfected with synthetic RNA derived from a prototype virus (H77-S) is described, which will substantially benefit antiviral and vaccine discovery programs.
Hepatitis C Virus Entry Depends on Clathrin-Mediated Endocytosis
TLDR
Postbinding cellular mechanisms of HCV entry were studied using both retroviral particles pseudotyped with HCV envelope glycoproteins and the HCV clone JFH-1, and data indicate that HCV enters target cells by clathrin-mediated endocytosis, followed by a fusion step from within an acidic endosomal compartment.
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