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Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei
TLDR
These experiments show that for mammals, nuclei from terminally differentiated, adult somatic cells of known phenotype introduced into enucleated oocytes are capable of supporting full development. Expand
Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer.
TLDR
It is found that 5-50 nM TSA-treatment for 10 h following oocyte activation resulted in more efficient in vitro development of somatic cloned embryos to the blastocyst stage from 2- to 5-fold depending on the donor cells including tail tip cells, spleen cells, neural stem cells, and cumulus cells. Expand
A Signaling Principle for the Specification of the Germ Cell Lineage in Mice
TLDR
This study establishes a robust strategy for reconstituting the mammalian germ cell lineage in vitro via Bmp4 signaling from the extraembryonic ectoderm, which is antagonized by the anterior visceral endoderm (AVE). Expand
Nuclear reprogramming of cloned embryos and its implications for therapeutic cloning
TLDR
The recent literature on nuclear reprogramming by SCNT is reviewed, including studies of gene expression, DNA methylation, chromatin remodeling, genomic imprinting and X chromosome inactivation, which seems to be highly efficient. Expand
Mice cloned from embryonic stem cells.
TLDR
It is shown that late-passage ES cells can be used to produce viable cloned mice and provide a link between the technologies of ES cells and animal cloning, suggesting that it may be possible to clone from a single cell a large number of individuals over an extended period. Expand
Development of normal mice from oocytes injected with freeze-dried spermatozoa
TLDR
The results suggest that it may be possible to store the male genomes at room temperature after freeze-drying mouse spermatozoa even after three month preservation in a dried state. Expand
Differentiation of Embryonic Stem Cell Lines Generated from Adult Somatic Cells by Nuclear Transfer
TLDR
These studies demonstrate the full pluripotency of ntES cells, which have contributed to an extensive variety of cell types, including dopaminergic and serotonergic neurons in vitro and germ cells in vivo. Expand
The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice.
TLDR
It is shown for the first time that by treating SCNT embryos with another HDACi, scriptaid, all the important inbred mouse strains can be cloned and the success of somatic nuclear reprogramming and cloning efficiency via nuclear transfer technique is clearly linked to the competent de novo synthesis of nascent mRNA in cloned mouse embryos. Expand
Mouse cloning with nucleus donor cells of different age and type
TLDR
The results indicate that adult somatic cells are not necessarily inferior to younger cells (fetal and ES cells) in the context of mouse cloning, although fetal cells are believed to have less genetic damage than adult Somatic cells. Expand
Stimulus-triggered fate conversion of somatic cells into pluripotency
TLDR
It is found that committed somatic cells give rise to STAP cells by reprogramming rather than selection, indicating that epigenetic fate determination of mammalian cells can be markedly converted in a context-dependent manner by strong environmental cues. Expand
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