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Glutamate transport into synaptic vesicles. Roles of membrane potential, pH gradient, and intravesicular pH.
- J. Tabb, P. Kish, R. V. Van Dyke, T. Ueda
- Chemistry, Medicine
- The Journal of biological chemistry
- 5 August 1992
It is concluded that optimal ATP-dependent glutamate uptake requires a large delta psi and a small delta pH, and that either delta psi,Delta pH, or a combination can drive glutamate uptake, but to different degrees. Expand
Glycolysis and Glutamate Accumulation into Synaptic Vesicles
It is demonstrated that the glycolytic enzymes glyceraldehyde phosphate dehydrogenase (GAPDH) and 3-phosphoglycerate kinase (3-PGK) are enriched in synaptic vesicles, forming a functional complex, and that synapticVesicles are capable of accumulating the excitatory neurotransmitter glutamate by harnessing ATP produced by vesicle-bound GAPDH/3- PGK at the expense of their substrates. Expand
Inhibition of vesicular glutamate storage and exocytotic release by Rose Bengal
The observations suggest that glutamate synaptic transmission could be altered by pharmacological intervention of glutamate uptake into synaptic vesicles in the nerve terminal, a new mode of synaptic manipulation for glutamate transmission. Expand
Phosphorylation of γ-aminobutyrate (GABA)/benzodiazepine receptors by cyclic AMP-dependent protein kinase
Preparations of gamma-aminobutyrate/benzodiazepine receptor from pig cerebral cortex are composed of three major bands of polypeptides which are purified in a ratio of approx. Expand
Active transport of gamma-aminobutyric acid and glycine into synaptic vesicles.
- P. Kish, C. Fischer-Bovenkerk, T. Ueda
- Chemistry, Medicine
- Proceedings of the National Academy of Sciences…
- 1 May 1989
Evidence is presented that GABA and glycine are each taken up into isolated synaptic vesicles in an ATP-dependent manner and that the uptake is driven by an electrochemical proton gradient. Expand
Glutamate transport and storage in synaptic vesicles.
A newly discovered, endogenous inhibitor of vesicular uptake, inhibitory protein factor (IPF), is discussed with some speculations as to its potential role as a presynaptic modulator of neurotransmission. Expand
Glutamate accumulation into synaptic vesicles.
This chapter describes the assay and properties of the glutamate transport system in the synaptic vesicle in three preparations: (1) bovine brain synapticvesicles highly purified by the use of antisynapsin I immunoglobulin G (IgG), (2) a bovin brain synapticVesicle fraction purified by sucrose density gradient centrifugation, and (3) a rat brain crude synaptic vESicle fraction. Expand
A large body of evidence now indicates that glutamate is specifically accumulated into synaptic vesicles, which provides strong support for the concept that glutamate was released from synaptic vESicles and is the major excitatory neurotransmitter. Expand
Characterization of the solubilized and reconstituted ATP-dependent vesicular glutamate uptake system.
The reconstitution experiments described here provide a functional assay for the solubilized vesicular glutamate uptake system and represent an initial step towards the purification of the glutamate translocator. Expand
Enzymatic oxidation. VII. Reduced diphosphopyridine nucleotide-rubredoxin reductase: properties and function as an electron carrier in hydroxylation.
DPNH is highly superior to TPNH as an electron donor and since two electron equivalents are accepted per mole of reductase, it is concluded that no active oxidation-reduction group is present other than the flavin. Expand