Share This Author
One molecule of diphtheria toxin fragment a introduced into a cell can kill the cell
Diphtheria toxin and related proteins. I. Isolation and properties of mutant proteins serologically related to diphtheria toxin.
Although Fragment A, which contains the enzymically active NH2-terminal region of the molecule, isolated from these mutant proteins could not be distinguished from Fragments A derived from toxin itself, cross-reacting materials 30 and 45 are nontoxic for susceptible animals and three mutant proteins are serologically indistinguishable from wild type toxin.
Reconstitution of Diphtheria Toxin from Two Nontoxic Cross-Reacting Mutant Proteins
The isolation of a new type of mutant Corynephage β, which carries a missense mutation in the structural gene for diphtheria toxin synthesis is described, which is immunologically indistinguishable from toxin itself but inhibits the action of toxin on HeLa cells, probably by competing for attachment sites on the cell membrane.
Isolation from corynebacterium diphtheriae C7(beta) of bacterial mutants that produce toxin in medium with excess iron
Findings show that the mutant strains are not phage mutants, but are bacterial host mutants, and that a host factor(s) is involved in the inhibition of toxin production by iron.
An immunological study of the diphtheria toxin molecule.
Mutation in the structural gene for diphtheria toxin carried by temperate phage .
Evidence suggesting that the toxin structural gene is part of the phage genome is described, suggesting that Corynebacterium diphtheriae strains lyso-genic for phage β are able to producediphtheria toxin.
Highly frequent single amino acid substitution in mammalian elongation factor 2 (EF-2) results in expression of resistance to EF-2-ADP-ribosylating toxins.
HVJ (Sendai virus)-induced envelope fusion and cell fusion are blocked by monoclonal anti-HN protein antibody that does not inhibit hemagglutination activity of HVJ.
Microinjection of T4 endonuclease V produced by a synthetic denV gene stimulates unscheduled DNA synthesis in both xeroderma pigmentosum and normal cells.