T. Tsuchiya

Publications325
h-index52
Citations9,972
Highly Influential Citations458
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NorM, a Putative Multidrug Efflux Protein, of Vibrio parahaemolyticus and Its Homolog in Escherichia coli
ABSTRACT We found that cells of Vibrio parahaemolyticus possess an energy-dependent efflux system for norfloxacin. We cloned a gene for a putative norfloxacin efflux protein from the chromosomal DNA… Expand
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Expression in Escherichia coli of a New Multidrug Efflux Pump, MexXY, from Pseudomonas aeruginosa
ABSTRACT Two new genes (mexXY) similar to mexAB,mexCD, and mexEF and mediating multidrug resistance were cloned from the chromosome of Pseudomonas aeruginosa. Elevated ethidium extrusion was observed… Expand
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Functional Cloning and Characterization of a Plant Efflux Carrier for Multidrug and Heavy Metal Detoxification*
We have identified a detoxifying efflux carrier from Arabidopsis using a functional cloning strategy. A bacterial mutant, KAM3, is deficient in multidrug resistance and does not survive on medium… Expand
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A putative multisubunit Na+/H+ antiporter from Staphylococcus aureus.
We cloned several genes encoding an Na+/H+ antiporter of Staphylococcus aureus from chromosomal DNA by using an Escherichia coli mutant, lacking all of the major Na+/H+ antiporters, as the host. E.… Expand
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Cloning and sequencing of the gene for Na+/H+ antiporter of Vibrio parahaemolyticus.
A gene encoding an Na+/H+ antiporter was cloned from vibrio parahaemolyticus into the plasmid pBR322 and expressed in Escherichia coli cells. The gene enabled mutant E. coli cells to grow in the… Expand
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Cloning and Sequencing of the Gene for Na+/H+Antiporter ofVibrio parahaemolyticus
Abstract A gene encoding an Na+/H+antiporter was cloned fromVibrio parahaemolyticusinto the plasmid pBR322 and expressed inEscherichia colicells. The gene enabled mutantE. colicells to grow in the… Expand
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Multidrug efflux transporters in the MATE family.
The MATE (Multidrug And Toxic Compound Extrusion) family is the most recently categorized one among five multidrug efflux transporter families. As far as we know, about twenty MATE transporters have… Expand
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A new Na+/H+ antiporter, NhaD, of Vibrio parahaemolyticus.
A gene encoding an Na+/H+ antiporter was cloned from chromosomal DNA of Vibrio parahaemolyticus, a slightly halophilic bacterium, and expressed in Escherichia coli cells. The gene enabled mutant E.… Expand
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AbeM, an H+-Coupled Acinetobacter baumannii Multidrug Efflux Pump Belonging to the MATE Family of Transporters
ABSTRACT We cloned a gene, abeM, for a multidrug efflux pump from Acinetobacter baumannii using Escherichia coli as the host. Sequence analysis revealed that AbeM is a member of the MATE family of… Expand
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Remarkable Synergies between Baicalein and Tetracycline, and Baicalein and β‐Lactams against Methicillin‐Resistant Staphylococcus aureus
During the screening of compounds that potentiate the effect of antimicrobial agents against methicillin‐resistant Staphylococcus aureus (MRSA), we found that an extract of thyme (Thymus vulgaris L)… Expand
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