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Genome structure and phylogenetic analysis of lettuce infectious yellows virus, a whitefly-transmitted, bipartite closterovirus.
TLDR
The complete nucleotide sequences of lettuce infectious yellows virus (LIYV) RNAs 1 and 2 are reported and it is suggested that the RdRp domain is expressed independently of the other replication-associated domains via a + 1 ribosomal frameshift.
Lettuce infectious yellows virus: in vitro acquisition analysis using partially purified virions and the whitefly Bemisia tabaci.
Virions of lettuce infectious yellows virus (LIYV; genus Crinivirus) were purified from LIYV-infected plants and their protein composition was analysed by SDS-PAGE and immunoblotting. Virion
Generation of cDNAs specific to lettuce infectious yellows closterovirus and other whitefly-transmitted viruses by RT-PCR and degenerate oligonucleotide primers corresponding to the closterovirus
TLDR
Oligonucleotide primers were designed based on nucleotide sequences corresponding to the conserved phosphate I and 2 motifs contained within the closterovirus-encoded heat shock protein 70 (HSP70) homolog and showed significant similarity to the HSP70 proteins in general and to the hSP70 homologs encoded byClosteroviruses in particular.
Asynchronous Accumulation of Lettuce Infectious Yellows Virus RNAs 1 and 2 and Identification of an RNA 1 trans Enhancer of RNA 2 Accumulation
TLDR
Time course and mutational analyses were used to examine the accumulation in protoplasts of progeny RNAs of the bipartiteCrinivirus, Lettuce infectious yellow virus and suggest that the LIYV RNA 1 ORF 2 encodes a trans enhancer for RNA 2 accumulation.
First Report of Cucurbit Yellow Stunting Disorder Virus (Genus Crinivirus) in North America.
TLDR
Definitive confirmation of CYSDV was obtained by performing reverse-transcription polymerase chain reaction (RT-PCR) analyses for two distinct CY SDV coding regions.
First Report of Tomato Brown Rugose Fruit Virus Infecting Greenhouse Tomato in the United States
TLDR
A new set of primers specific to ToBRFV was designed and validated to have no cross-reaction to ToMV and ToMMV, and was confirmed on the inoculated plants using RT-PCR and amplicon sequencing.
Comparative cytopathology of Crinivirus infections in different plant hosts
TLDR
The main cytopathological effects induced in plants by criniviruses were common in young leaves and included alterations of the chloroplasts and the presence of BYV-type inclusion bodies in companion cells.
Molecular and Biological Characterization of Tomato mottle mosaic virus and Development of RT-PCR Detection.
TLDR
The characterization in molecular and biological properties of ToMMV would provide fundamental knowledge to manage this emerging virus on tomato and other solanaceous crops in the U.S. and around the world.
Resistance of Tetranychus urticae Koch (Acari: Tetranychidae) to Cyhexatin and Fenbutatin-Oxide in California Pears
TLDR
Although correlation between resistance to cy hexatin and fenbutatin-oxide among 39 colonies was significant, lack of a change in cyhexatin resistance after fen butatin- oxide application in the field suggests multiple resistance to these compounds.
First Report of Tobacco rattle virus in Spinach in California.
TLDR
In 2009 in coastal California, commercially grown spinach in two nearby fields exhibited symptoms of a previously unrecognized virus-like disease that was identified as TRV, the first report of TRV in spinach in California.
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