Quantitative targeted absolute proteomics of human blood–brain barrier transporters and receptors
- Yasuo Uchida, S. Ohtsuki, T. Terasaki
- Biology, ChemistryJournal of Neurochemistry
- 1 April 2011
J. Neurochem. (2011) 117, 333–345.
Simultaneous Absolute Protein Quantification of Transporters, Cytochromes P450, and UDP-Glucuronosyltransferases as a Novel Approach for the Characterization of Individual Human Liver: Comparison…
- S. Ohtsuki, O. Schaefer, T. Terasaki
- BiologyDrug Metabolism And Disposition
- 1 January 2012
Findings indicate that protein expression levels determined by the present simultaneous quantification method are a useful parameter to assess differences of hepatic function between individuals.
Contribution of Carrier-Mediated Transport Systems to the Blood–Brain Barrier as a Supporting and Protecting Interface for the Brain; Importance for CNS Drug Discovery and Development
- S. Ohtsuki, T. Terasaki
- BiologyPharmaceutical Research
- 10 July 2007
The blood–brain barrier (BBB) forms an interface between the circulating blood and the brain and possesses various carrier-mediated transport systems for small molecules to support and protect CNS…
The Blood–Brain Barrier Creatine Transporter is a Major Pathway for Supplying Creatine to the Brain
- S. Ohtsuki, M. Tachikawa, T. Terasaki
- BiologyJournal of Cerebral Blood Flow and Metabolism
- 1 November 2002
The authors examined the contribution made by the creatine transporter (CRT) at the blood–brain barrier in supplying creatine to the brain from blood and found that CRT is expressed in TM-BBB cells and isolated mouse brain microvessels.
Quantitative Atlas of Membrane Transporter Proteins: Development and Application of a Highly Sensitive Simultaneous LC/MS/MS Method Combined with Novel In-silico Peptide Selection Criteria
- J. Kamiie, S. Ohtsuki, T. Terasaki
- Biology, ChemistryPharmaceutical Research
- 25 January 2008
A sensitive and simultaneous quantification method was developed for membrane proteins that constructed a quantitative atlas of membrane transporter proteins at the blood–brain barrier, liver and kidney in mouse.
Brain efflux index as a novel method of analyzing efflux transport at the blood-brain barrier.
- A. Kakee, T. Terasaki, Y. Sugiyama
- Biology, ChemistryJournal of Pharmacology and Experimental…
- 1 June 1996
Results demonstrate that the brain efflux index method is a useful technique for analyzing an efflux process from the brain across the blood-brain barrier involving a carrier-mediated transport system.
Transcriptomic and quantitative proteomic analysis of transporters and drug metabolizing enzymes in freshly isolated human brain microvessels.
- R. Shawahna, Yasuo Uchida, J. Scherrmann
- Biology, MedicineMolecular Pharmaceutics
- 8 July 2011
The extensive investigation of gene and protein patterns of transporters and metabolizing enzymes provides new molecular information for understanding drug entry and metabolism in the human blood-brain barrier.
Quantitative atlas of blood-brain barrier transporters, receptors, and tight junction proteins in rats and common marmoset.
- Yutaro Hoshi, Yasuo Uchida, M. Tachikawa, Takashi Inoue, S. Ohtsuki, T. Terasaki
- BiologyJournal of Pharmacy and Science
- 1 September 2013
The marmoset may be a good model to access in vivo human BBB permeability characteristics, as an alternative to rat and macaque monkey, and also to investigate inter-species and inter-strain differences across rodents and primates.
Large‐scale multiplex absolute protein quantification of drug‐metabolizing enzymes and transporters in human intestine, liver, and kidney microsomes by SWATH‐MS: Comparison with MRM/SRM and HR‐MRM/PRM
- Kenji Nakamura, Mio Hirayama-Kurogi, S. Ohtsuki
- 1 August 2016
The results indicate that SWATH‐MS enables large‐scale multiplex absolute protein quantification while retaining similar quantitative capability to MRM/SRM or HR‐MRM/PRM.
A pericyte‐derived angiopoietin‐1 multimeric complex induces occludin gene expression in brain capillary endothelial cells through Tie‐2 activation in vitro
- Satoko Hori, S. Ohtsuki, K. Hosoya, E. Nakashima, T. Terasaki
- BiologyJournal of Neurochemistry
- 1 April 2004
In vitro BBB model studies revealed that the pericyte‐derived multimeric angiopoietin‐1/Tie‐2 pathway induces occludin expression, derived from astrocytes and pericytes that ensheathe brain microvessels.