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Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.
Many cancer cells consume large quantities of glutamine to maintain TCA cycle anaplerosis and support cell survival. It was therefore surprising when RNAi screening revealed that suppression ofExpand
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An RNA 5′-Triphosphatase Related to the Protein Tyrosine Phosphatases
mRNA capping requires the sequential action of three enzymatic activities: RNA triphosphatase, guanylyl-transferase, and methyltransferase. Here we characterize a gene (CEL-1) believed to encode theExpand
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Efficient Synthesis of 13C,15N-Labeled RNA Containing the Cap Structure m7GpppA
Studying the mechanism by which the cap structure performs its numerous biological roles in mRNA metabolism by NMR spectroscopy requires the large-scale production of isotopically labeled capped RNA.Expand
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Live-Cell Imaging of Caspase Activation for High-Content Screening
Caspases are central to the execution of programmed cell death, and their activation constitutes the biochemical hallmark of apoptosis. In this article, the authors report the successful adaptationExpand
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A fluorescence-based thiol quantification assay for ultra-high-throughput screening for inhibitors of coenzyme A production.
Here we report the development and miniaturization of a cell-free enzyme assay for ultra-high-throughput screening (uHTS) for inhibitors of two potential drug targets for obesity and cancer: fattyExpand
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The Caenorhabditis elegans mRNA 5'-capping enzyme. In vitro and in vivo characterization.
Eukaryotic mRNA capping enzymes are bifunctional, carrying both RNA triphosphatase (RTPase) and guanylyltransferase (GTase) activities. The Caenorhabditis elegans CEL-1 capping enzyme consists of anExpand
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Divergent Subunit Interactions among Fungal mRNA 5′-Capping Machineries
ABSTRACT The Saccharomyces cerevisiae mRNA capping enzyme consists of two subunits: an RNA 5′-triphosphatase (RTPase) and GTP::mRNA guanylyltransferase (GTase). The GTase subunit (Ceg1) binds to theExpand
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Comparison of luminescence ADP production assay and radiometric scintillation proximity assay for Cdc7 kinase.
Several assay technologies have been successfully adapted and used in HTS to screen for protein kinase inhibitors; however, emerging comparative analysis studies report very low hit overlap betweenExpand
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Validation of a high-content screening assay using whole-well imaging of transformed phenotypes.
Automated microscopy was introduced two decades ago and has become an integral part of the discovery process as a high-content screening platform with noticeable challenges in executing cell-basedExpand
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Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase
SummaryThe antibody to 65 KD mycobacterial heat shock protein (HSP65) and antibody to human superoxide dismutase (H-SOD) were measured by ELISA in patients with autoimmune hepatitis (AIH), andExpand
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