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Substrate binding to the peripheral site of acetylcholinesterase initiates enzymatic catalysis. Substrate inhibition arises as a secondary effect.
TLDR
It is shown that cationic substrates such as acetylthiocholine and 7-acetoxy-N-methylquinolinium (M7A) bind to the peripheral site as well as to the acylation site, which indicates that the primary physiologic role of the AChE peripheral site is to accelerate the hydrolysis of acetylcholine at low substrate concentrations. Expand
Nonequilibrium analysis alters the mechanistic interpretation of inhibition of acetylcholinesterase by peripheral site ligands.
TLDR
An alternative nonequilibrium analysis of the steady-state inhibition patterns of the active site gorge of acetylcholinesterase is introduced, which concludes that this model can account for the inhibition of AChE by small peripheral site ligands such as propidium without invoking any conformational interaction between the peripheral and acylation sites. Expand
Biochemical and subcellular changes in carp exposed to the organophosphorus methidathion and the pyrethroid deltamethrin
TLDR
The observed changes in AChE indicate how serious the neuronal damage is, while the other parameters relate to the stressed state of the fish. Expand
Similarities and differences between the massive eel (Anguilla anguilla L.) devastations that occurred in Lake Balaton in 1991 and 1995.
TLDR
In the past few years, two massive eel devastations occurred in Lake Balaton, Hungary, and investigations carried out to find the causes included measurements of certain biochemical parameters, indicating damage to different muscle tissues to an extent never observed previously. Expand
Acetylthiocholine binds to asp74 at the peripheral site of human acetylcholinesterase as the first step in the catalytic pathway.
TLDR
D74 is important in conveying to the acylation site an inhibitory conformational effect induced by the binding of fasciculin to the peripheral site, and this inhibitory effect is measured by the relative decrease in the first-order phosphorylation rate constant k(OP) for the neutral organophosphate 7-[(methylethoxyphosphonyl)oxy]-4-methylcoumarin (EMPC). Expand
A steric blockade model for inhibition of acetylcholinesterase by peripheral site ligands and substrate.
TLDR
The nonequilibrium kinetic analysis is employed to extend the steric blockade model to include blockade of the dissociation of substrate hydrolysis products by bound peripheral site ligand and reports that acetylthiocholine can bind to the AChE peripheral site with an equilibrium dissociation constant K(S) of about 1 mM. Expand
In vivo effects of deltamethrin exposure on activity and distribution of molecular forms of carp AChE.
TLDR
In vivo effects of the insecticide deltamethrin on the activity and molecular forms of acetylcholinesterase (AChE, EC 3.1.7) were examined in different organs of carp, and no other tissues investigated exhibited any changes in the distribution of the AChE molecular forms. Expand
Relationship Between the Structure of Some Humic Compounds and Their Inhibitory Effects on Carp Catalase
TLDR
Ever more studies have been published concerning the enzyme-inhibitory effects of plantphenols in algae, bacteria, plants and mammals (Goldstein and Swain 1965; Wetzel 1992, 1993; Erdei et al. 1994; Reed 1995). Expand
Organophosphorylation of Acetylcholinesterase in the Presence of Peripheral Site Ligands
TLDR
In contrast, the binding of the polypeptide neurotoxin fasciculin to the peripheral site of AChE dramatically decreased phosphorylation rate constants, suggesting that molecular or electronic strain caused by the proximity of propidium to EMPC in the ternary complex may promoteosphorylation. Expand
In vivo effects of deltamethrin on some biochemical parameters of carp (Cyprinus carpio L.)
TLDR
Thein vivo effects of deltamethrin (DM) on the blood sugar level, the acetylcholinesterase (AChE) activities of the blood serum and various organs, and the catalase activity of the liver were examined to demonstrate the effect of DM on different fish enzymes, at low concentrations under laboratory conditions, which might be useful in practice for biomonitoring using fish. Expand
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