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Modification of Fatty Acid Composition in Rice Plants by Transformation with a Tobacco Microsomal ω-3 Fatty Acid Desaturase Gene (NtFAD3)
A tobacco microsomal ω-3 fatty acid desaturase gene (NtFAD3) under the control of CaMV35S promoter was introduced into rice plants by the microprojectile-mediated transformation. The transgenic… Expand
Improvement of environmental stress tolerance of sweet potato by introduction of genes for spermidine synthase
With the purpose of enhancing environmental stress tolerance of sweet potato (Ipomoea batatas, cv. Kokei 14), we transformed this plant with spermidine synthase genes derived from Cucurbita ficifolia… Expand
Transgenic Plant Production from Embryogenic Callus of Sweet Potato (Ipomoea batatas (L.) Lam.) Using Agrobacterium tumefaciens
Transformed sweet potato plants were obtained from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA101/pIG121-Hm used in the present study… Expand
Production of transgenic wheat through particle bombardment of scutellar tissues: Frequency is influenced by culture duration
Summary A transformation system for hexaploid wheat was developed by using scutellar tissues of cultured immature embryos via particle bombardment. The scutellar tissues of immature embryos were… Expand
Variation in transformation frequencies among six common wheat cultivars through particle bombardment of scutellar tissues.
The transformation technique in common wheat has already been established by using microprojectile bombardment and scutellar tissues of immature embryos. In this study, in vitro culture response of… Expand
Does Decrease in Ribulose-1,5-Bisphosphate Carboxylase by Antisense RbcS Lead to a Higher N-Use Efficiency of Photosynthesis under Conditions of Saturating CO2 and Light in Rice Plants?
Rice (Oryza sativa L.) plants with decreased ribulose-1,5-bisphosphate carboxylase (Rubisco) were obtained by transformation with the rice rbcS antisense gene under the control of the rice rbcS… Expand
Co-integration, co-expression and co-segregation of an unlinked selectable marker gene and NtFAD3 gene in transgenic rice plants produced by particle bombardment.
Using the scutellar tissue of rice mature embryos as a target tissue, a selectable marker gene, bar, and an unselectable gene, fatty acid desaturase gene from tobacco (NtFAD3), on separate plasmids,… Expand
Effect of six promoter-intron combinations on transient reporter gene expression in einkorn, emmer and common wheat cells by particle bombardment
Abstract The effect of six promoter-intron combinations on transient expression of β-glucuronidase (GUS) as the reporter gene was estimated in cultured einkorn ( Triticum monococcum ), emmer (… Expand
Increase of amylose content of sweetpotato starch by RNA interference of the starch branching enzyme II gene (IbSBEII)
In the storage roots of sweetpotato (Ipomoea batatas (L.) Lam. cv. Kokei 14), 10 to 20% of the starch is essentially unbranched linear amylose and the other major component is branched amylopectin.… Expand
Efficient embryogenic callus formation in sweet potato (Ipomoea batatas (L.) Lam.)
Shoot apices of sweet potato (Ipomoea batatas (L.) Lam.) were cultured on LS medium containing 3 % (W/V) sucrose, 0.32 % (W/V) gellan gum and various concentrations (O, 0.5, 1, 2, 4 mg/l ) of eight… Expand