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Identification of a Biosynthetic Gene Cluster in Rice for Momilactones*
Functional analysis by heterologous expression in Escherichia coli followed by enzyme assays demonstrated that the AK103462 protein catalyzes the conversion of 3β-hydroxy-9βH-pimara-7,15-dien-19,6β-olide into momilactone A, providing strong evidence for the presence on chromosome 4 of a gene cluster involved in momILactone biosynthesis.
Fusicoccins are biosynthesized by an unusual chimera diterpene synthase in fungi
It is found that (+)-fusicocca-2,10 (14)-diene, a tricyclic hydrocarbon precursor for fusicoccins, is biosynthesized from the C5 isoprene units by an unusual multifunctional enzyme, P. amygdali fusICoccadiene synthase (PaFS), which shows both prenyltransferase and terpene cyclase activities.
Diterpene Cyclases Responsible for the Biosynthesis of Phytoalexins, Momilactones A, B, and Oryzalexins A–F in Rice
Rice (Oryza sativa L.) produces diterpene phytoalexins, such as momilactones, oryzalexins, and phytocassanes. Using rice genome information and in vitro assay with recombinant enzymes, we identified
Effects of combined treatment with rapamycin and cotylenin A, a novel differentiation-inducing agent, on human breast carcinoma MCF-7 cells and xenografts
Combined treatment with rapamycin and cotylenin A significantly inhibited the growth of MCF-7 cells as xenografts, without apparent adverse effects, suggesting that this combination may have therapeutic value in treating breast cancer.
Erinacine Q, a New Erinacine from Hericium erinaceum, and its Biosynthetic Route to Erinacine C in the Basidiomycete
The search for new cyathane metabolites from a liquid culture of Hericium erinaceum YB4-6237 resulted in the isolation of a new erinacine named erinACine Q, which was determined to be a derivative in which the formyl group of er inacine P had been reduced to the hydroxymethyl group.
Characterization of a Rice Gene Family Encoding Type-A Diterpene Cyclases
Re recombinant OsKS5 and OsKS6 expressed in E. coli converted ent-copalyl diphosphate into ent-pimara-8(14),15-diene and ent-kaur-15-ene, respectively, suggest that the diterpene cyclase genes responsible for gibberellin and phytoalexin biosynthesis are not functionally redundant.
Cloning of a Gene Cluster Responsible for the Biosynthesis of Diterpene Aphidicolin, a Specific Inhibitor of DNA Polymerase α
This study shows that simple PCR-based genome walking without constructing a genomic DNA library is useful for identification of a small gene cluster and proposes a general strategy for the cloning the biosynthetic genes of fungal diterpenoids by using fungal GGS.
Functional Analysis of the Two Interacting Cyclase Domains in ent-Kaurene Synthase from the FungusPhaeosphaeria sp. L487 and a Comparison with Cyclases from Higher Plants*
Kinetic analysis and identification of the two cyclase domains in a bifunctional diterpene cyclase, Phaeosphaeria ent-kaurene synthase (FCPS/KS) indicate that there are two separate interacting domains in the 106-kDa polypeptide of FCPS/ KS.
Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis.
The results strongly suggest the presence of two ent-CDP synthase isoforms in rice, one that participates in the biosynthesis of GAs and a second that is involved in the BIOSynthesis of phytoalexins.
Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a
A cDNA encoding a novel diterpene cyclase, OsDTC1, is isolated and characterized from suspension-cultured rice cells treated with a chitin elicitor to investigate the regulatory mechanisms of the biosynthesis of (-)-phytocassanes in rice.