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Pectin, pectinase and protopectinase: production, properties, and applications.
TLDR
Research has been initiated with the aim of developing an improved alternative microbial pectin production process by means of a specific enzyme, called “protopectinase,” which solubilizes protopectin forming highly polymerized soluble pECTin. Expand
Purification and properties of two type-B alpha-L-arabinofuranosidases produced by Penicillium chrysogenum.
TLDR
Two distinct extracellular alpha-L-arabinofuranosidases (AFases; EC 3.55) were purified from the culture filtrate of Penicillium chrysogenum 31B and displayed broad substrate specificities; they released arabinose from branched arabinan, debranchedArabinan), arabinoxylan, arabinogalactan, and arabino-oligosaccharides. Expand
Enzymatic high digestion of soybean milk residue (okara).
TLDR
It was found that two pectinases could digest the secondary cell wall of okara, and the final residues were identified as oil body complexes in the soybean cells and fiber-like organ between the cells. Expand
Identification of a GH62 α-l-arabinofuranosidase specific for arabinoxylan produced by Penicillium chrysogenum
TLDR
Results suggest that AXS5, an arabinoxylan arabinofuranohydrolase isolated by in vitro cloning, is involved in xylan degradation. Expand
Esterification of ferulic acid with polyols using a ferulic acid esterase from Aspergillus niger.
TLDR
The enzyme responsible for the esterification was purified and characterized, and was found to be quite similar to an A. niger ferulic acid esterase (FAE-III) in terms of molecular mass, pH and temperature optima, substrate specificity on synthetic substrates, and the N-terminal amino acid sequence. Expand
Purification and characterisation of two exo-polygalacturonases from Aspergillus niger able to degrade xylogalacturonan and acetylated homogalacturonan.
TLDR
Two exo-polygalacturonases were purified from a commercial Aspergillus niger enzyme preparation by ammonium sulfate precipitation, preparative electrofocusing, anion-exchange and size-exclusion chromatographies and revealed that both enzymes split the substrate from the non-reducing end in a multi-chain attack mode. Expand
Synthesis of highly water-soluble feruloyl diglycerols by esterification of an Aspergillus niger feruloyl esterase
TLDR
Water-soluble FA derivatives were synthesized by esterification of FA with diglycerol (DG) using feruloyl esterase purified from a commercial enzyme preparation produced by Aspergillus niger, with major reaction product FA-DG1 determined to be γ-feruloysl-α,α′-DGs by NMR and electrospray ionization mass spectrometry analyses. Expand
Expression of Aspergillus oryzae phytase gene in Aspergillus oryzae RIB40 niaD(-).
TLDR
The culture broth of the transformant, which was grown in a medium containing starch as a carbon source and polyvinylpyrrolidone showed phytase activity of a maximum of 2.0 units ml(-1) at 37 degrees C, pH 5.5. Expand
Purification, Characterization, and Overexpression of Thermophilic Pectate Lyase of Bacillus sp. RN1 Isolated from a Hot Spring in Thailand
TLDR
Unsaturated di- and trigalacturonic acids were formed mainly as the final products of degradation by Pel SWU, as revealed by high-performance anion-exchange chromatography (HPAEC) and electrospray ionization mass spectrometry (ESI-MS) analyses. Expand
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