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Purification and properties of two type-B alpha-L-arabinofuranosidases produced by Penicillium chrysogenum.
Two distinct extracellular alpha-L-arabinofuranosidases (AFases; EC 3.2.1.55) were purified from the culture filtrate of Penicillium chrysogenum 31B. The molecular masses of the enzymes wereExpand
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Enzymatic high digestion of soybean milk residue (okara).
The objective of this study was to digest okara in high yield by food-processing enzymes. Autoclaving of okara was effective in increasing cellulase digestion for the primary cell wall, and theExpand
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Purification, Characterization, and Overexpression of Thermophilic Pectate Lyase of Bacillus sp. RN1 Isolated from a Hot Spring in Thailand
A thermophilic pectate lyase, Pel SWU, was isolated from a culture filtrate of Bacillus sp. RN1 isolated from a hot spring in Ranong Province, Thailand. The enzyme was purified to homogeneity usingExpand
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Identification of a GH62 α-l-arabinofuranosidase specific for arabinoxylan produced by Penicillium chrysogenum
An arabinoxylan arabinofuranohydrolase (AXS5) was purified from the culture filtrate of Penicillium chrysogenum 31B. A cDNA encoding AXS5 (axs5) was isolated by in vitro cloning using the N-terminalExpand
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Purification, characterization and gene cloning of two forms of a thermostable endo-xylanase from Streptomyces sp. SWU10
Abstract Two forms of an endo-xylanase were isolated from the culture filtrate of Streptomyces sp. SWU10 that grew on rice straw. The molecular masses of both forms of an enzyme were 31 kDa (XynSW2A)Expand
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Immunostimulatory Activity of Polysaccharides Isolated from Caulerpa lentillifera on Macrophage Cells
Polysaccharides were extracted from Caulerpa lentillifera by treating with water and then purified by size-exclusion chromatography. The purified polysaccharides, termed SP1, were found to beExpand
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Esterification of ferulic acid with polyols using a ferulic acid esterase from Aspergillus niger.
Commercially available enzyme preparations were screened for enzymes that have a high ability to catalyze direct ester-synthesis of ferulic acid with glycerol. Only a preparation, Pectinase PLExpand
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Purification and characterisation of two exo-polygalacturonases from Aspergillus niger able to degrade xylogalacturonan and acetylated homogalacturonan.
Two exo-polygalacturonases (EC 3.2.1.67) were purified from a commercial Aspergillus niger enzyme preparation by ammonium sulfate precipitation, preparative electrofocusing, anion-exchange andExpand
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Synthesis of highly water-soluble feruloyl diglycerols by esterification of an Aspergillus niger feruloyl esterase
Ferulic acid (FA) is a component of plant cell walls that has applications in food, cosmetic, and health products, but its applications are limited by its high insolubility. We synthesizedExpand
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Expression of Aspergillus oryzae phytase gene in Aspergillus oryzae RIB40 niaD(-).
Aspergillus oryzae RIB40 niaD(-) was transformed using a plasmid constructed with the A. oryzae phytase gene and pNAN8142 vector. The culture broth of the transformant, which was grown in a mediumExpand
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