Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.
Results suggest that the recombinant Ad efficiently expressed functional Cre and offers a basis for establishing a powerful on/off switching strategy of gene expression in cultured mammalian cells and presumably in transgenic animals.
Quantitative Analysis of UDP-Glucuronosyltransferase (UGT) 1A and UGT2B Expression Levels in Human Livers
This study comprehensively determined the absolute values of mRNA expression of each UGT isoform in human livers and found considerable interindividual variability.
Aryl hydrocarbon receptor suppresses intestinal carcinogenesis in ApcMin/+ mice with natural ligands
- K. Kawajiri, Yasuhito Kobayashi, Y. Fujii‐Kuriyama
- Biology, ChemistryProceedings of the National Academy of Sciences
- 11 August 2009
It is shown that the AhR E3 ubiquitin ligase has a role in suppression of intestinal carcinogenesis by a previously undescribed ligand-dependent β-catenin degradation pathway that is independent of and parallel to the APC system.
Multiple forms of human P450 expressed in Saccharomyces cerevisiae. Systematic characterization and comparison with those of the rat.
Avian Cytochrome P450 (CYP) 1-3 Family Genes: Isoforms, Evolutionary Relationships, and mRNA Expression in Chicken Liver
All of the existing isoforms of CYP1-3 in avian species using available genome assemblies are clarified and classify, suggesting that avian xenobiotic-metabolizing CYP genes have undergone unique evolution such as CYP2C and CYP3A genes, which have undergone avian-specific gene duplications.
Metabolism of vitamin D3 by cytochromes P450.
Analysis of the metabolites of vitamin D3 by the reconstituted system, CYP27A1 surprisingly produced at least seven forms of minor metabolites including 1alpha,25( OH)2D3 in addition to the major metabolite 25(OH)D3, indicating that human CYP 27A1 catalyzes multiple reactions involved in the vitamin D 3 metabolism.
Intestinal and Hepatic CYP3A4 Catalyze Hydroxylation of 1α,25-Dihydroxyvitamin D3: Implications for Drug-Induced Osteomalacia
Induction of CYP3A4-dependent 1,25(OH)2D3 metabolism by antiepileptic drugs and other PXR ligands may diminish intestinal effects of the hormone and contribute to osteomalacia.
Molecular Cloning and Characterization of CYP719, a Methylenedioxy Bridge-forming Enzyme That Belongs to a Novel P450 Family, from cultured Coptis japonica Cells*
- Nobuhiro Ikezawa, Masaru Tanaka, F. Sato
- Biology, ChemistryJournal of Biological Chemistry
- 3 October 2003
Two cytochrome P450 cDNAs involved in the biosynthesis of berberine, an antimicrobial benzylisoquinoline alkaloid, were isolated from cultured Coptis japonica cells and characterized and heterologous expression in yeast confirmed that it had the same activity as a methylenedioxy bridge-forming enzyme (canadine synthase).
Metabolism of vitamin D by human microsomal CYP2R1.
No enzyme activity of 25-hydroxyvitamin D3 1alpha-hydroxylase gene product in pseudovitamin D deficiency rickets, including that with mild clinical manifestation.
- S. Kitanaka, A. Murayama, S. Kato
- BiologyJournal of Clinical Endocrinology and Metabolism
- 1 November 1999
Results indicate that all of the patients, including those of mild phenotype, are caused by 1alpha-hydroxylase gene mutations that totally abolish the enzyme activity.