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Biophysical characterizations of human mitochondrial transcription factor A and its binding to tumor suppressor p53
Human mitochondrial transcription factor A (TFAM) is a multi-functional protein, involved in different aspects of maintaining mitochondrial genome integrity. In this report, we characterized TFAM andExpand
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Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution.
Sequence saturation mutagenesis (SeSaM) is a conceptually novel and practically simple method that truly randomizes a target sequence at every single nucleotide position. A SeSaM experiment can beExpand
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A statistical analysis of random mutagenesis methods used for directed protein evolution.
We have developed a statistical method named MAP (mutagenesis assistant program) to equip protein engineers with a tool to develop promising directed evolution strategies by comparing 19 mutagenesisExpand
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Sensitive Assay for Laboratory Evolution of Hydroxylases toward Aromatic and Heterocyclic Compounds
Powerful directed evolution methods have been developed for tailoring proteins to our needs in industrial applications. Here, the authors report a medium-throughput assay system designed forExpand
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Physical and functional interactions between human mitochondrial single-stranded DNA-binding protein and tumour suppressor p53
Single-stranded DNA-binding proteins (SSB) form a class of proteins that bind preferentially single-stranded DNA with high affinity. They are involved in DNA metabolism in all organisms and serve aExpand
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The diversity challenge in directed protein evolution.
Over the past decade, we have witnessed a bloom in the field of evolutive protein engineering which is fueled by advances in molecular biology techniques and high-throughput screening technology.Expand
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Protein engineering in bioelectrocatalysis.
Electrochemistry of redox proteins is a broadly applicable technology with important applications in biosensors, biofuel cells and chemical syntheses. Escalating attention in this area is driven byExpand
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Structural and dynamic properties of cytochrome P450 BM‐3 in pure water and in a dimethylsulfoxide/water mixture
Solvent molecules play an important role for the structural and dynamical properties of proteins. A major focus of current protein engineering is the development of enzymes that are catalyticallyExpand
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Carbon Dioxide Capture and Utilization using Biological Systems: Opportunities and Challenges
Carbon dioxide capture and utilization (CCU), a concept of turning a greenhouse gas into a useful feedstock, is gaining much attention in recent years. Increasing CO2 emission into the atmosphere,Expand
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The accessory subunit of mitochondrial DNA polymerase γ determines the DNA content of mitochondrial nucleoids in human cultured cells
The accessory subunit of mitochondrial DNA polymerase γ, POLGβ, functions as a processivity factor in vitro. Here we show POLGβ has additional roles in mitochondrial DNA metabolism. Mitochondrial DNAExpand
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