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Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences.
A new primer design strategy for PCR amplification of unknown targets that are related to multiply-aligned protein sequences that consists of a short 3' degenerate core region and a longer 5' consensus clamp region, which demonstrates the practical utility of this hybrid primer method. Expand
Detection and analysis of diverse herpesviral species by consensus primer PCR
Estimation of template dilution studies in the presence of human carrier DNA demonstrated that six human herpesviruses could be detected at levels at or below 100 genome equivalents per 100 ng of carrier DNA, suggesting that consensus primer PCR targeted to herpesviral DNA polymerase may prove to be useful in the detection and identification of known herpesvirus in clinical samples and the initial characterization of new herpesViral genomes. Expand
Plaque-associated expression of human herpesvirus 6 in multiple sclerosis.
Examination of 86 brain specimens by PCR demonstrated that HHV-6 was present in > 70% of MS cases and controls and is thus a commensal virus of the human brain, suggesting an association of HHv-6 with the etiology or pathogenesis of MS. Expand
CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design
The primer design software and the CODEHOP PCR strategy have been utilized for the identification and characterization of new gene orthologs and paralogs in different plant, animal and bacterial species and has been successful in identifying new pathogen species. Expand
Type C Retrovirus Released from Porcine Primary Peripheral Blood Mononuclear Cells Infects Human Cells
It is shown here that mitogenic activation of peripheral blood mononuclear cells isolated from the National Institutes of Health miniature pig and the Yucatan pig resulted in the activation and release of an infectious type C retrovirus. Expand
Identification of two homologs of the Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) in retroperitoneal fibromatosis of different macaque species
DNA sequence evidence for two new herpesviruses closely related to KSHV from RF tissues of two macaque species, Macaca nemestrina and Macaca mulatta suggest a new group within the subfamily Gammaherpesvirinae whose members are implicated in the pathogenesis of KS and KS-like neoplasms in different primate species. Expand
Integrin αVβ3 Binds to the RGD Motif of Glycoprotein B of Kaposi's Sarcoma-Associated Herpesvirus and Functions as an RGD-Dependent Entry Receptor
It is demonstrated that αVβ3 is a cellular receptor mediating both the cell adhesion and entry of KSHV into target cells through binding the virion-associated gB(RGD). Expand
Evolution of the acyl-CoA binding protein (ACBP).
Experimental evidence indicates that the function of ACBP has been conserved from yeast to humans and that the multiple lineage-specific paralogues have evolved altered functions. Expand
Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6.
  • T. Rose, A. Bruce
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 October 1991
It is proposed that OSM, LIF, G-CSF, and IL-6 are structurally related members of a cytokine family that have in common the ability to modulate differentiation of a variety of cell types. Expand
ATP scavenging by the intracellular pathogen Porphyromonas gingivalis inhibits P2X7‐mediated host‐cell apoptosis
Primary gingival epithelial cells, an important component of the oral innate immune response, also express functional P2X7 and are sensitive to ATP‐induced apoptosis, and NDK promotes survival of host cells by hydrolysing extracellular ATP and preventing apoptosis‐mediated through P2x7. Expand