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Genome Streamlining in a Cosmopolitan Oceanic Bacterium
TLDR
P. ubique, the first cultured member of the SAR11 clade, has the smallest genome and encodes the smallest number of predicted open reading frames known for a free-living microorganism. Expand
Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite
TLDR
A metagenomic analysis of the bacterial community resident in the hindgut paunch of a wood-feeding ‘higher’ Nasutitermes species shows the presence of a large, diverse set of bacterial genes for cellulose and xylan hydrolysis, the first system-wide gene analysis of a microbial community specialized towards plant lignocellulose degradation. Expand
The genome of Nanoarchaeum equitans: Insights into early archaeal evolution and derived parasitism
TLDR
The hyperthermophile Nanoarchaeum equitans is an obligate symbiont growing in coculture with the crenarchaeon Ignicoccus, and represents a basal archaeal lineage and has a highly reduced genome. Expand
Domestication and Divergence of Saccharomyces cerevisiae Beer Yeasts
TLDR
The genomes and phenomes of 157 industrial Saccharomyces cerevisiae yeasts are presented to shed light on the origins, evolutionary history, and phenotypic diversity of industrial yeasts and provide a resource for further selection of superior strains. Expand
Metagenomes from High-Temperature Chemotrophic Systems Reveal Geochemical Controls on Microbial Community Structure and Function
TLDR
Multiple-pathway and protein-specific functional analysis of metagenome sequence data corroborated results from phylogenetic analyses and clearly demonstrate major differences in metabolic potential across sites. Expand
Exploring Nitrilase Sequence Space for Enantioselective Catalysis
TLDR
137 unique nitrilases, discovered from screening of >600 biotope-specific environmental DNA (eDNA) libraries, were characterized, providing a catalytic toolbox for enantiospecific synthesis of a variety of carboxylic acid derivatives, as well as an intriguing library for evolutionary and structural analyses. Expand
A universal approach to the expression of human and rabbit cytochrome P450s of the 2C subfamily in Escherichia coli.
TLDR
This work has identified important factors required for the high-level expression of 2C subfamily P450s in E. coli and the availability of these enzymes will facilitate detailed kinetic measurements for known and yet to be identified substrates. Expand
Phylogenetic Distribution of CRISPR-Cas Systems in Antibiotic-Resistant Pseudomonas aeruginosa
TLDR
It is demonstrated that CRISPR-Cas systems play an important role in shaping the accessory genomes of globally distributed P. aeruginosa strains while also providing substantial data for subsequent genomic and experimental studies in multiple fields. Expand
Microsomal P450 2C3 is expressed as a soluble dimer in Escherichia coli following modification of its N-terminus.
TLDR
A variant of P450 2C3 was constructed that lacked the putative membrane-spanning segment of the N-terminus of microsomal P450s, and this enzyme could be purified in the absence of detergent using immobilized metal affinity chromatography following extraction from isolated membranes in high salt buffers. Expand
A Novel, High Performance Enzyme for Starch Liquefaction
TLDR
The synergistic approach of combining environmental discovery and laboratory evolution for identification and optimization of industrially important biocatalysts with exceptional process compatibility and economics is described. Expand
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