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Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
EVIDENCE FOR RECYCLING OF SYNAPTIC VESICLE MEMBRANE DURING TRANSMITTER RELEASE AT THE FROG NEUROMUSCULAR JUNCTION
During stimulation the intracellular compartments of this synapse change shape and take up extracellular protein in a manner which indicates that synaptic vesicle membrane added to the surface during exocytosis is retrieved by coated vesicles and recycled into new synaptic vESicles by way of intermediate cisternae.
FINE STRUCTURAL LOCALIZATION OF A BLOOD-BRAIN BARRIER TO EXOGENOUS PEROXIDASE
These findings localize, at a fine structural level, a "barrier" to the passage of peroxidase at the endothelium of vessels in the cerebral cortex in mice, particularly with reference to a recent study in which similar techniques were applied to capillaries in heart and skeletal muscle.
JUNCTIONS BETWEEN INTIMATELY APPOSED CELL MEMBRANES IN THE VERTEBRATE BRAIN
Endothelial and epithelial tight junctions occlude the interspaces between blood and parenchyma or cerebral ventricles, thereby constituting a structural basis for the blood-brain and blood-cerebrospinal fluid barriers.
Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release
- J. Heuser, T. Reese, M. Dennis, Y. Jan, L. Jan, L. Evans
- BiologyThe Journal of cell biology
- 1 May 1979
The utility of quick- freezing as a technique to capture biological processes as evanescent as synaptic transmission as well as physiological demonstrations that quanta are discharged independently has been established.
Persistent Accumulation of Calcium/Calmodulin-Dependent Protein Kinase II in Dendritic Spines after Induction of NMDA Receptor-Dependent Chemical Long-Term Potentiation
Green fluorescent protein (GFP)-CaMKIIα translocation into synaptic spines during NMDA receptor-dependent chemical LTP (cLTP) is measured and found that under these conditions, translocation is persistent, consistent with the hypothesis that CaMKII α accumulation at synapses is a memory trace of past synaptic activity.
Different axoplasmic proteins generate movement in opposite directions along microtubules in vitro
Mass of the postsynaptic density and enumeration of three key molecules.
- Xiaobing Chen, L. Vinadé, T. Reese
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 9 August 2005
The total molecular mass of individual postsynaptic densities (PSDs) isolated from rat forebrain was measured by scanning transmission EM and mass contributions of PSD-95, synapse-associated protein (SAP)97, and alpha-Ca2+/calmodulin-dependent protein kinase II (CaMKII) were determined by quantitative gel electrophoresis ofPSD fractions.
Organization of the core structure of the postsynaptic density
- Xiaobing Chen, C. Winters, T. Reese
- BiologyProceedings of the National Academy of Sciences
- 18 March 2008
EM tomography is used to delineate the organization of PSDs at glutamatergic synapses in rat hippocampal cultures and shows that PSD-95 is a component of these filaments.