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Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
TLDR
The partial purification of a protein from squid giant axons and optic lobes that induces microtubule-based movements is reported and there is a homologous protein in bovine brain, for which the name kinesin is proposed. Expand
EVIDENCE FOR RECYCLING OF SYNAPTIC VESICLE MEMBRANE DURING TRANSMITTER RELEASE AT THE FROG NEUROMUSCULAR JUNCTION
TLDR
During stimulation the intracellular compartments of this synapse change shape and take up extracellular protein in a manner which indicates that synaptic vesicle membrane added to the surface during exocytosis is retrieved by coated vesicles and recycled into new synaptic vESicles by way of intermediate cisternae. Expand
FINE STRUCTURAL LOCALIZATION OF A BLOOD-BRAIN BARRIER TO EXOGENOUS PEROXIDASE
TLDR
These findings localize, at a fine structural level, a "barrier" to the passage of peroxidase at the endothelium of vessels in the cerebral cortex in mice, particularly with reference to a recent study in which similar techniques were applied to capillaries in heart and skeletal muscle. Expand
JUNCTIONS BETWEEN INTIMATELY APPOSED CELL MEMBRANES IN THE VERTEBRATE BRAIN
TLDR
Endothelial and epithelial tight junctions occlude the interspaces between blood and parenchyma or cerebral ventricles, thereby constituting a structural basis for the blood-brain and blood-cerebrospinal fluid barriers. Expand
Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release
TLDR
The utility of quick- freezing as a technique to capture biological processes as evanescent as synaptic transmission as well as physiological demonstrations that quanta are discharged independently has been established. Expand
Mass of the postsynaptic density and enumeration of three key molecules.
TLDR
The total molecular mass of individual postsynaptic densities (PSDs) isolated from rat forebrain was measured by scanning transmission EM and mass contributions of PSD-95, synapse-associated protein (SAP)97, and alpha-Ca2+/calmodulin-dependent protein kinase II (CaMKII) were determined by quantitative gel electrophoresis ofPSD fractions. Expand
Persistent Accumulation of Calcium/Calmodulin-Dependent Protein Kinase II in Dendritic Spines after Induction of NMDA Receptor-Dependent Chemical Long-Term Potentiation
TLDR
Green fluorescent protein (GFP)-CaMKIIα translocation into synaptic spines during NMDA receptor-dependent chemical LTP (cLTP) is measured and found that under these conditions, translocation is persistent, consistent with the hypothesis that CaMKII α accumulation at synapses is a memory trace of past synaptic activity. Expand
Different axoplasmic proteins generate movement in opposite directions along microtubules in vitro
TLDR
Results indicate that there is a retrograde bead translocator which is pharmacologically and immunologically distinct from kinesin, a direction that corresponds to anterograde transport in the axon. Expand
Organelle, bead, and microtubule translocations promoted by soluble factors from the squid giant axon
TLDR
A reconstituted system for examining directed organelle movements along purified microtubules has been developed and found that these movements may be driven by a single, soluble ATPase that binds reversibly to organelles, beads, or glass and generates a translocating force on a microtubule. Expand
Organization of the core structure of the postsynaptic density
TLDR
EM tomography is used to delineate the organization of PSDs at glutamatergic synapses in rat hippocampal cultures and shows that PSD-95 is a component of these filaments. Expand
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