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Methods for estimating lipid peroxidation: an analysis of merits and demerits.
The merits and demerits of various assays to measure lipid peroxidation are analyzed and certain restraints should be considered while selecting the method for estimation.
A conformational analysis of Walker motif A [GXXXXGKT (S)] in nucleotide-binding and other proteins.
An examination of the immediate neighborhood of the Walker sequence indicates that this region is preceded by a beta-strand and followed by an alpha-helix, resulting in the motif beta-W-alpha, an invariant feature amongst nucleotide-binding proteins.
Generation of H2O2 in biomembranes
Knowledge of the generation of H202 in cellular oxidations has existed for many years. It has been assumed that H202 is tOxiC tO cells and the presence of catalase is indicative of a detoxication
Vanadate-stimulated NADH oxidation in plasma membrane.
The rate of NADH oxidation with oxygen as the acceptor is very low in mouse liver plasma membrane and erythrocyte membrane, but when vanadate is added, this rate is stimulated 10- to 20-fold, indicating that oxygen is the electron acceptor and the reaction is mediated by superoxide.
Inhibition of mitochondrial oxidative phosphorylation by adriamycin.
The antitumour antibiotic, adriamycin, inhibited oxidative phosphorylation in freshly prepared mitochondria from the heart, liver and kidney of the rat, and was extremely sensitive to the drug when hexokinase was present in the reaction medium.
Purification and properties of fructose diphosphate aldolase from spinach leaves.
Fructose 1,6-diphosphate aldolase has been purified from spinach leaves and appears to contain three active sites, estimated by reduction of the Schiff base intermediate with borohydride.
Inhibition of rat liver mevalonate pyrophosphate decarboxylase and mevalonate phosphate kinase by phenyl and phenolic compounds.
Of the three mevalonate-metabolizing enzymes, meValonate pyrophosphate decarboxylase has the lowest specific activity and is probably the rate-determining step in this part of the pathway.
New insights of superoxide dismutase inhibition of pyrogallol autoxidation
By dismutating oxygen radicals, an action it is known for, SOD can reverse autoxidation, echoing the reported proposal of superoxide:semiquinone oxidoreductase activity for SOD.
Heat exposure and hypothyroid conditions decrease hydrogen peroxide generation in liver mitochondria.
The results indicate that H2O2 generation in mitochondria of heat-exposed animals is determined by thyroid status, and treatment of rats with thyroxine stimulated H2 O2 generation by a mechanism apparently involving fresh protein synthesis.