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ACTION OF X-RAYS ON MAMMALIAN CELLS
The effects of x-irradiation have been quantitatively studied on single cells of a human cervical carcinoma (HeLa) under conditions such that 100 per cent of the unirradiated cells reproduce inExpand
GENETICS OF SOMATIC MAMMALIAN CELLS III. LONG-TERM CULTIVATION OF EUPLOID CELLS FROM HUMAN AND ANIMAL SUBJECTS
A methodology designed to eliminate mitotic inhibitor action and involving use of pretested fetal calf serum and careful pH and temperature control has been described by which cells from normal humanExpand
ACTION OF X-RAYS ON MAMMALIAN CELLS
Survival curves of normal human cells from a variety of tissues exposed to varying doses of x-irradiation have been constructed, which permit definition of the intrinsic radiation sensitivity of theExpand
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
Two methods for simple and rapid plating of single HeLa cells, human, carcinomatous cells, are described. These result in growth and formation of colonies from each single cell. One of theseExpand
LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION.
Equations are presented describing the accumulation of cells at any part of the life cycle as a result of addition of specific blocking agents. An experimental methodology using these relationshipsExpand
Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.
  • F. Kao, T. Puck
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 August 1968
LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. II. CELLS FROM THE CHINESE HAMSTER OVARY GROWN IN SUSPENSION CULTURE.
A method for life cycle analysis in mammalian cells which utilizes the collection function has been applied to the Chinese hamster ovary grown in suspension. The following durations were found forExpand
THE LETHAL EFFECT OF RELATIVE HUMIDITY ON AIR-BORNE BACTERIA
  • E. Dunklin, T. Puck
  • Chemistry, Medicine
  • The Journal of experimental medicine
  • 31 January 1948
The viability of pneumococcus, Type I, sprayed into the atmosphere from a liquid suspension was measured as a function of the relative humidity. When broth, saliva, or 0.5 per cent saline solution isExpand
GENETICS OF SOMATIC MAMMALIAN CELLS : II. CHROMOSOMAL CONSTITUTION OF CELLS IN TISSUE CULTURE
A convenient, reliable method for chromosome delineation of animal cells grown as monolayers on glass has been applied to human, opossum, and Chinese hamster cells. Tissue cultured cells from 5Expand
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