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High-resolution crystal structure of cytochrome P450cam.
The crystal structure of Pseudomonas putida cytochrome P450cam with its substrate, camphor, bound has been refined to R = 0.19 at a normal resolution of 1.63 A. While the 1.63 A model confirms our
Structure of a cytochrome P450-redox partner electron-transfer complex.
TLDR
Substantial differences between theFMN-binding domains of P450BM-3 and microsomal P450 reductase, observed around the flavin-binding sites, are responsible for different redox properties of the FMN, which, in turn, control electron flow to the P450.
Crystal structure of horseradish peroxidase C at 2.15 Å resolution
TLDR
The crystal structure of horseradish peroxidase isozyme C (HRPC) has been solved to 2.15 Å resolution and the key residue involved in direct interactions with aromatic donor molecules is identified, predicted to be important for the ability of HRPC to bind aromatic substrates.
Engineered ascorbate peroxidase as a genetically-encoded reporter for electron microscopy
TLDR
The development of 'APEX', a genetically encodable EM tag that is active in all cellular compartments and does not require light, is reported, a monomeric 28-kDa peroxidase that withstands strong EM fixation to give excellent ultrastructural preservation.
The structure of the cytochrome p450BM-3 haem domain complexed with the fatty acid substrate, palmitoleic acid
TLDR
A comparison of the substrate-bound and -free forms reveals major conformational differences and provides the first detailed picture of substrate-induced conformational changes in a P450.
The crystal structure of peanut peroxidase.
TLDR
This is the first complete structure of a class III peroxidase and as such should serve as a model for other class III enzymes including the much-studied horseradish per oxidase.
The 2.6-A crystal structure of Pseudomonas putida cytochrome P-450.
The crystal structure of Pseudomonas putida cytochrome P-450cam in the ferric, camphor bound form has been determined and partially refined to R = 0.23 at 2.6 A. The single 414 amino acid polypeptide
Crystal structure of cytochrome P450 14α-sterol demethylase (CYP51) from Mycobacterium tuberculosis in complex with azole inhibitors
TLDR
Mapping mutations identified in Candida albicans azole-resistant isolates indicates that azole resistance in fungi develops in protein regions involved in orchestrating passage of CYP51 through different conformational stages along the catalytic cycle rather than in residues directly contacting fluconazole.
The crystal structure of manganese peroxidase from Phanerochaete chrysosporium at 2.06-A resolution.
TLDR
The crystal structure of manganese peroxidase (MnP) from the lignin-degrading basidiomycetous fungus Phanerochaete chrysosporium has been solved using molecular replacement techniques and a new cation binding site was located in the crystal structure.
Crystal Structure of Constitutive Endothelial Nitric Oxide Synthase A Paradigm for Pterin Function Involving a Novel Metal Center
TLDR
The unexpected recognition of the substrate, L-arginine, at the H4B site indicates that this site is poised to stabilize a positively charged pterin ring and suggests a model involving a cationic pterIn radical in the catalytic cycle.
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