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A specific interaction between coat protein and helper component correlates with aphid transmission of a potyvirus.
Specific binding between the coat protein (CP) and the helper component (HC) of the tobacco vein mottling potyvirus (TVMV) was characterized using a protein blotting-overlay protocol. In this inExpand
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Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility.
  • C. D. Atreya, T. Pirone
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 15 December 1993
We have previously provided evidence that amino acid substitutions within the N-terminal portion of the helper component-proteinase (HC-Pro) from tobacco vein mottling virus (TVMV), in particular atExpand
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Variations in the VPg protein allow a potyvirus to overcome va gene resistance in tobacco.
The va gene is used in commercial Burley tobacco cultivars including cv TN 86 to confer resistance to tobacco vein mottling virus (TVMV) and, to some extent, other potyviruses. A naturally occurringExpand
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Site-directed mutations in the potyvirus HC-Pro gene affect helper component activity, virus accumulation, and symptom expression in infected tobacco plants.
Helper component (HC-Pro) is a virus-encoded nonstructural protein required for aphid transmission of potyviruses. In the tobacco vein mottling virus (TVMV) polyprotein, HC-Pro represents a 457Expand
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Mutations in the potyvirus helper component protein: effects on interactions with virions and aphid stylets.
Mutations of K --> E in the highly conserved 'KITC' motif of the potyvirus helper component (HC) protein result in loss of HC function in aphid transmission, presumably because of inability toExpand
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A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus.
A nonaphid transmissible (NAT) variant of tobacco vein mottling virus (TVMV) was used to test the hypothesis that the viral coat protein (CP) plays a role in determining aphid transmissibility.Expand
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Amino acid substitutions in the coat protein result in loss of insect transmissibility of a plant virus.
Amino acids near the N terminus of the coat protein of tobacco vein mottling virus were deleted or altered by site-directed mutagenesis to determine the effect on aphid transmissibility of the virus.Expand
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Helper-dependent vector transmission of plant viruses.
A variety of noncirculatively transmitted viruses have evolved a vector transmission strategy that involves, in addition to virions, virus-encoded proteins that are not constituents of virions. TheseExpand
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Aphid stylet activities during potyvirus acquisition from plants and anin vitro system that correlate with subsequent transmission
The behavioural events associated with acquisition of tobacco etch potyvirus by starvedMyzus persicae during single, electrically-recorded penetrations of plants or a Parafilm membrane were compared.Expand
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Spontaneous mutagenesis of a plant potyvirus genome after insertion of a foreign gene.
The RNA genome of tobacco etch potyvirus (TEV) was engineered to express bacterial beta-glucuronidase (GUS) fused to the virus helper component proteinase (HC-Pro). It was shown previously thatExpand
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