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Collagen hydroxylases and the protein disulfide isomerase subunit of prolyl 4-hydroxylases.
This review concentrates on recent progress toward understanding the detailed mechanism of 4-hydroxylase action, including: occurrence and function of the enzyme in animals; general molecular properties; and intracellular sites of hydroxylation. Expand
Lack of collagen XVIII/endostatin results in eye abnormalities
The findings provide an explanation for high myopia, vitreoretinal degeneration and retinal detachment seen in patients with Knobloch syndrome caused by loss‐of‐function mutations in collagen XVIII. Expand
Generation of biologically active endostatin fragments from human collagen XVIII by distinct matrix metalloproteases.
The results indicate that certain, especially cancer-related, MMP family members can generate biologically active endostatin-containing polypeptides from collagen XVIII and thus, by releasing endstatin fragments, may participate in the inhibition of endothelial cell proliferation, migration and angiogenesis. Expand
VEGFR-3 and its ligand VEGF-C are associated with angiogenesis in breast cancer.
The results suggest that VEGF-C secreted by the intraductal carcinoma cells acts predominantly as an angiogenic growth factor for blood vessels, although this paracrine signaling network between the cancer cells and the endothelium may also be involved in modifying the permeabilities of both blood and lymphatic vessels and metastasis formation. Expand
Two new collagen subgroups: membrane-associated collagens and types XV and XVII.
It is hoped that gene “knockout” experiments in transgenic mice and the introduction of dominant-negative collagen mutations into mice will result in increased understanding of the function of these collagens, and the categorization of all known members of the collagen family into subgroups is discussed. Expand
Interaction of endostatin with integrins implicated in angiogenesis.
It is demonstrated that recombinantly produced human endostatin interacts with alpha(5)- and alpha(v)-integrins on the surface of human endothelial cells, and the interaction is of functional significance in vitro, as it is found that immobilizedendostatin supports endothelial cell survival and migration in an integrin-dependent manner. Expand
Assembly of human prolyl 4‐hydroxylase and type III collagen in the yeast Pichia pastoris: formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen
The data indicate that collagen synthesis in Pichia, and probably also in other cells, involves a highly unusual control mechanism, in that production of a stable prolyl 4‐hydroxylase requires collagen expression while assembly of astable collagen requires enzyme expression. Expand
Site‐directed mutagenesis of human protein disulphide isomerase: effect on the assembly, activity and endoplasmic reticulum retention of human prolyl 4‐hydroxylase in Spodoptera frugiperda insect
Report here on the expression in baculovirus vectors of various mutant PDI/beta‐subunits together with a wild‐type alpha‐subunit of the human prolyl 4‐hydroxylase alpha 2 beta 2 tetramer in Spodoptera frugiperda insect cells. Expand
Characterization of the human prolyl 4-hydroxylase tetramer and its multifunctional protein disulfide-isomerase subunit synthesized in a baculovirus expression system.
Prolyl 4-hydroxylase (EC, an alpha 2 beta 2 tetramer, catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The enzyme can easily be dissociated into its subunits,Expand
Molecular cloning of the beta‐subunit of human prolyl 4‐hydroxylase. This subunit and protein disulphide isomerase are products of the same gene.
The product of a single gene appears to possess two different enzymatic functions depending on whether it is present in cells in monomer form or in the prolyl 4‐hydroxylase tetramer. Expand